Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Thermus thermophilus HB8

ABSTRACT: Thermus thermophilus small crRNAs isolated from the T. thermophilus CMR complex

PROVIDER: PRJNA218552 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR975070_1.fastq.gz	Fastqsanger.gz
	SRR975070_2.fastq.gz	Fastqsanger.gz
	SRR975071_1.fastq.gz	Fastqsanger.gz
	SRR975071_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Thermus thermophilus HB8

Project description:Thermus thermophilus small crRNAs isolated from the T. thermophilus CMR complex

| PRJNA215364 | ENA

Thermus thermophilus HB8

Project description:Thermus thermophilus HB8

| PRJNA161933 | ENA

Expression profile of crp-deficient Thermus thermophilus HB8 strain

Project description:We observed the expression profile of the total mRNA of crp (TTHA1437) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70 degC

2010-10-15 | GSE21459 | GEO

Expression data from Thermus thermophilus HB8 wild type and TTHA1559 disrupted

Project description:We observed the expression profile of the total mRNA of wild-type and TTHA1559 disrupted Thermus thermophilus HB8 strain at 70°C.

2014-08-03 | GSE33985 | GEO

TTHB212 (anti-sigmaE)-deficient Thermus thermophilus HB8 strain: time course

Project description:We observed the expression profile of the total mRNA of TTHB212-deficient of Thermus thermophilus HB8 strain grown in a rich medium at 70°C for 7 and 8 hours

2010-10-15 | GSE19758 | GEO

A genome-wide view of the expression and processing patterns of Thermus thermophilus HB8 CRISPR RNAs

Project description:The CRISPR-Cas system represents an RNA-based adaptive immune response system in prokaryotes. CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) consist of arrays of short repeat sequences interspaced by non-repetitive short spacers, some of which show sequence similarity to foreign phage genetic elements. Their cistronic transcripts are processed to produce the mature CRISPR RNAs (crRNAs), the elements that confer immunity by base-pairing with exogenous nucleic acids. We characterized the expression and processing patterns of Thermus thermophilus HB8 CRISPRs using differential deep-sequencing, which differentiates between 5’ monophosphate and 5’ non-monophosphate-containing RNAs, and/or between 3’ hydroxyl and 3’ non-hydroxyl-containing RNAs. The genome of T. thermophilus HB8 encodes 11 CRISPRs, classified into three distinct repeat sequence types, all of which were constitutively expressed without deliberately infecting the bacteria with phage. Analysis of the differential deep sequencing data suggested that crRNAs are generated by endonucleolytic cleavage, leaving fragments with 5’ hydroxyl and 3’ phosphate or 2’,3’-cyclic phosphate termini. The 5’ ends of all crRNAs are generated by site-specific cleavage eight nucleotides upstream of the spacer start position, however, the 3’ ends, are generated by two alternative, repeat-sequence-type-dependent mechanisms. These observations are consistent with the operation of multiple crRNA processing systems within a bacterial strain.

2012-02-25 | GSE34328 | GEO

Thermus thermophilus HB8

Project description:Thermus thermophilus HB8 Raw sequence reads

| PRJNA1084259 | ENA

Thermus thermophilus HB8

Project description:Expression profile of TTHA0252-deficient Thermus thermophilus HB8 strain

| PRJNA129525 | ENA

Thermus thermophilus HB8

Project description:Expression profile of TTHA0655-deficient Thermus thermophilus HB8 strain

| PRJNA129727 | ENA

Thermus thermophilus HB8

Project description:Expression profile of TTHA0167-deficient Thermus thermophilus HB8 strain