Project description:Microarray analysis of exosomal and cellular mRNAs in activated and resting primary human T lymphoblasts Primary human T lymphoblasts were obtained from eight donors and total RNA was isolated from cells and derived exosomes before and after activation with PMA plus ionomycin.
Project description:Microarray analysis of exosomal and cellular miRNAs in activated and resting primary human T lymphoblasts Primary human T lymphoblasts were obtained from eight donors and total RNA was isolated from cells and derived exosomes before and after activation with PMA plus ionomycin.
Project description:miRNA profiling of resting and activated T cells Two condition experiment, resting versus activated T cells, measured pooled samples from three independent stimulations
Project description:Macrophage-derived exosomes hold significant promise for clinical disease therapy. Macrophages can be categorized into several subtypes. The resting state is known as the M0 subtype, and the exosomes they secrete are termed M0-Exo. Macrophages can be polarized to the M2 subtype by combined induction with IL-4 and IL-13, and the exosomes derived from M2 macrophages are referred to as M2-Exo. miRNAs are important cargo within exosomes. Since both M0-Exo and M2-Exo exhibit therapeutic effects in inflammatory responses, we performed miRNA sequencing on the contents of M0-Exo and M2-Exo to investigate the underlying mechanisms of their therapeutic actions.
Project description:We hypothesized that miRNAs in the bone maroow mesenchymal stem cells (BM-MSC)-derived exosomes contributed to the phenotype change of breast cancer cells through exosome transfer. We analyzed the miRNA expression signature in BM-MSC-derived exosomes. We compared the miRNA expression levels in exosomes between BM-MSCs and adult fibroblasts (as a control). In this study, miRNA expression including in bone-marrow mesenchymal cell (BM-MSC)-derived exosomes was examined, and compared with that of exosomes derived from adult fibroblast cells or the BM-MSC cells. In addition, miRNA expression of BM-MSC exosomes was also compared with that of breast cancer cells with or without cancer stem cell marker.