Project description:This is global mRNA gene expression data for HEK293 and cells expressing HSP70K71E ; most genes were down-regulated for HSP70K71E compared to the control Keywords: Global mRNA expression
Project description:<p>Translation fidelity is the limiting factor in the accuracy of gene expression. With an estimated frequency of 10-4, errors in mRNA decoding occur in a mostly stochastic manner. Little is known about the response of higher eukaryotes to chronic loss of ribosomal accuracy as per an increase in the random error rate of mRNA decoding. Here, we present a global and comprehensive picture of the cellular changes in response to translational accuracy in mammalian ribosomes impaired by genetic manipulation. In addition to affecting established protein quality control pathways, such as elevated transcript levels for cytosolic chaperones, activation of the ubiquitin-proteasome system, and translational slowdown, ribosomal mistranslation led to unexpected responses. In particular, we observed increased mitochondrial biogenesis associated with import of misfolded proteins into the mitochondria and silencing of the unfolded protein response in the endoplasmic reticulum.</p><p><br></p><p>This study describes the metabolomic analysis of HEK293 cells lines expressing mutant ribosomal protein RPS2 (human A226Y). RPS2 A226Y mutation has been shown to cause misreading and readthrough. Results provide insight into the response to chronic mistranslation in mammalian cells.</p>
Project description:This is global mRNA gene expression data for HEK293 and cells expressing HSP70K71E ; most genes were down-regulated for HSP70K71E compared to the control Keywords: Global mRNA expression We overexpressed pCI1-EGFP (control) and Hsp70K71E-EGFP to HEK293 and collected total RNA from each condition to analyze global mRNA expression
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived transcriptome profiling of Wild Type and RBM7-/- HEK293 cell lines and RNA immunoprecipitation (RIP) of Flag-tagged RBM7-expressing HEK293 cells
Project description:Methylated DNA immunoprecipitation-sequencing of HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1)
