Flavobacterium columnare
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ABSTRACT:
PROVIDER: PRJNA226721 | ENA |
REPOSITORIES: ENA
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Project description:Clinical Flavobacterium columnare ATCC 49512 was grown on Flavobacterium columnare growth medium (FCGM). Bacteria from four colonies at mid-exponential phase were harvested, total proteins were isolated, and identified using 2-DE MALDI TOF/TOF MS and 2-D LC ESI MS/MS analyses. The MS/MS spectra for all peptides were analyzed using sequest algorithm
2016-03-11 | PRD000810 | Pride
Project description:Flavobacterium columnare, the causative agent of columnaris disease causes substantial mortality worldwide in numerous freshwater finfish species. Due to its global significance and impact on the aquaculture industry continual efforts to better understand basic mechanisms that contribute to disease are urgently needed. F. columnare naturally occurs in a planktonic, free living state where it can survive for long periods of time, even in the absence of nutrients. In contrast, F. columnare also possesses the ability to form biofilms, broadly defined as surface bound microbial communities inhabiting an organic matrix composed of autogenously derived extracellular polymeric substances. The advantages of adopting this life stage are not completely clear for F. columnare, but biofilm formation could increase virulence by offering protection from desiccation, augment resistance to antimicrobials, improve nutrient acquisition, and protection against other bacteria. To examine gene expression between F. columnare planktonic cells and biofilms, we conducted a study where both phases were grown with and without stimulation and then sampled for RNA sequencing.
2018-10-16 | GSE109937 | GEO
Project description:Catfish farming remains number one among U.S. aquaculture production. Flavobacterium columnare, the causative agent of columnaris disease, produces substantial mortality during the production of freshwater farmed finfish species. F. columnare is ubiquitous in the aquatic environment, and outbreaks of disease are often triggered during the spring and summer months of the catfish production cycle. As food fish production continues to increase, the frequency of columnaris disease will only continue to rise within the aquaculture industry and thus new preventative measures will be required. Vaccination against different fish pathogens has proven to be an effective disease management practice within the aquaculture industry. To evaluate the immunological effect of bath immunization on catfish with a recombinant F. columnare DNAk protein vaccine, we performed RNA sequencing on skin samples from control and rDNAK immunized catfish at different time intervals. We evaluated the different gene expression patterns between the groups with a focus on identifying differences in innate and adaptive immune function.
2019-06-20 | GSE121116 | GEO
Project description:Flavobacterium columnare Genome sequencing
| PRJNA1082490 | ENA
Project description:Flavobacterium columnare Genome sequencing
| PRJNA1228342 | ENA
Project description:Flavobacterium columnare Genome sequencing
| PRJNA358757 | ENA
Project description:Catfish farming remains number one among U.S. aquaculture production. Flavobacterium columnare, the causative agent of columnaris disease, produces substantial mortality during the production of freshwater farmed finfish species. F. columnare is ubiquitous in the aquatic environment, and outbreaks of disease are often triggered during the spring and summer months of the catfish production cycle. As food fish production continues to increase, the frequency of columnaris disease will only continue to rise within the aquaculture industry and thus new preventative measures will be required. Copper sulfate is the most common algaecide used in aquaculture. Copper sulfate has also been suggested as a therapeutant for columnaris disease, as we have previously discovered copper sulfate treatment effects the survivability and gill histology of channel catfish during an infection with F. columnare. Therefore to evaluate the functional effects of treatment with copper sulfate on columnaris disease, we performed RNA sequencing on gill samples from control and copper sulfate-treated catfish either immediately (more susceptible) or 24 hours after (more resistant) copper treatment followed by a challenge with F. columnare. We evaluated the differential gene expression patterns over the time-course of infection between groups with a focus on identifying functional differences in immune function.
2026-08-05 | GSE136222 | GEO
Project description:Flavobacterium columnare Genome sequencing and assembly
| PRJNA360885 | ENA
Project description:Flavobacterium columnare Genome sequencing and assembly
| PRJNA431138 | ENA