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Homo sapiens

ABSTRACT: Profiling of miRNA expression of HeLa cells infected with Salmonella Typhimurium

OTHER RELATED OMICS DATASETS IN: E-GEOD-53280

PROVIDER: PRJNA231558 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1049354.fastq.gz	Fastqsanger.gz
	SRR1049355.fastq.gz	Fastqsanger.gz
	SRR1049356.fastq.gz	Fastqsanger.gz
	SRR1049357.fastq.gz	Fastqsanger.gz
	SRR1049358.fastq.gz	Fastqsanger.gz

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Homo sapiens

Project description:Transcriptomic profiling of HeLa cells infected with Salmonella Typhimurium

| PRJNA231559 | ENA

miRNA expression analysis of Salmonella Typhimurium infected cells

Project description:To have a global picture of the miRNAs regulated upon Salmonella infection, we assessed small RNA changes, by RNA-sequencing, of HeLa cells infected with Salmonella Typhimurium compared with mock-treated cells . In addtion to the total population, we evaluated miRNA expression in the fraction of HeLa cells with internalized bacteria (Salmonella-positive), as well as in bystander cells, separated by fluorescence activated cell sorting (FACS)

2021-04-12 | GSE147362 | GEO

Profiling of miRNA expression of HeLa cells infected with Salmonella Typhimurium

Project description:We identified miRNAs differentially regulated upon Salmonella infection by comparative deep-sequencing analysis of cDNA libraries prepared from the small RNA population (10M-bM-^@M-^S29 nt) of HeLa cells infected with Salmonella (20 hpi) and mock-treated cells. Considering that at a MOI of 25 Salmonella is internalized in only 10-15% of the HeLa cells, we separated the fraction of cells which had internalized Salmonella (Salmonella+) from the bystander fraction (Salmonella-) by fluorescence-activated cell sorting (FACS), and extended the analysis of miRNA changes to these samples. Interestingly, we observed that Salmonella infection induces a significant decrease in the expression of all the detected members of the miR-15 family miRNA profiles of HeLa cells infected with Salmonella Typhimurium were generated by deep sequencing, using Illumina HiSeq2000.

2014-07-18 | E-GEOD-53278 | biostudies-arrayexpress

Proteomic analysis of the secretome of Salmonella-infected Hela cells

Project description:It is becoming increasingly clear that cells infected with pathogens can signal to bystander cells. Infected cells have the ability to alert and instruct bystander cells to mount pro-inflammatory cytokine response, thus contributing to clearing infections. Here we analyse secretome of HeLa cells infected with Salmonella enterica serovar Typhimurium strain SL1344. Cells were infected with a MOI of 100 for 14 hours. Secretome from mock- and Salmonella-infected cells was collected for mass-spectrometry analysis.

2021-04-16 | PXD018026 | Pride

Transcriptomic profiling of HeLa cells infected with Salmonella Typhimurium

Project description:We evaluated the transcriptome changes induced by infection with Salmonella (20 hpi, MOI 100). Transcriptmic profiles of HeLa cells infected with Salmonella Typhimurium were generated by deep sequencing, using Illumina HiSeq 2000.

2014-07-18 | E-GEOD-53280 | biostudies-arrayexpress

E-GEOD-53280

Project description:Transcriptomic profiling of HeLa cells infected with Salmonella Typhimurium

2025-01-24 | E-GEOD-53280 | ExpressionAtlas

miRNA expression analysis of Salmonella Typhimurium infected cells

Project description:miRNA expression analysis of Salmonella Typhimurium infected cells

| PRJNA614015 | ENA

Homo sapiens immortalized MSCs: 3A6

Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.

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SopD2-UltraID-HA_Styphimurium-HeLa_Brumell_2025

Project description:Dataset contains files from HeLa cells infected with UltraID-SopD2-HA-expressing Salmonella typhimurium. Cells were supplied with biotin and lysed in modified RIPA buffer. Lysates were incubated with streptavidin sepharose and captured proteins were digested with trypsin. Samples were analyzed on TIMS-TOF operated in positive ESI mode (DDA). LC was performed on an EvosepOne instrument (SPD30).

2025-08-19 | MSV000098876 | MassIVE

Profiling of miRNA expression of HeLa cells infected with Salmonella Typhimurium

Project description:We identified miRNAs differentially regulated upon Salmonella infection by comparative deep-sequencing analysis of cDNA libraries prepared from the small RNA population (10–29 nt) of HeLa cells infected with Salmonella (20 hpi) and mock-treated cells. Considering that at a MOI of 25 Salmonella is internalized in only 10-15% of the HeLa cells, we separated the fraction of cells which had internalized Salmonella (Salmonella+) from the bystander fraction (Salmonella-) by fluorescence-activated cell sorting (FACS), and extended the analysis of miRNA changes to these samples. Interestingly, we observed that Salmonella infection induces a significant decrease in the expression of all the detected members of the miR-15 family

2014-07-18 | GSE53278 | GEO

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