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E-GEOD-53280

ABSTRACT: Transcriptomic profiling of HeLa cells infected with Salmonella Typhimurium

OTHER RELATED OMICS DATASETS IN: PRJNA231559PRJNA231558PRJNA231557PRJNA231560

PROVIDER: E-GEOD-53280 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-53280-analysis-methods.tsv	Tabular
	E-GEOD-53280-analytics.tsv	Tabular
	E-GEOD-53280-analytics.tsv.undecorated	Tabular
	E-GEOD-53280-analytics.tsv.undecorated.unrounded	Tabular
	E-GEOD-53280-analytics.tsv.unrounded	Tabular

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Project description:Transcriptomic profiling of HeLa cells infected with Salmonella Typhimurium

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miRNA expression analysis of HeLa cells treated with secretome of Salmonella infected cells

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miRNA expression analysis of Salmonella Typhimurium infected cells

Project description:To have a global picture of the miRNAs regulated upon Salmonella infection, we assessed small RNA changes, by RNA-sequencing, of HeLa cells infected with Salmonella Typhimurium compared with mock-treated cells . In addtion to the total population, we evaluated miRNA expression in the fraction of HeLa cells with internalized bacteria (Salmonella-positive), as well as in bystander cells, separated by fluorescence activated cell sorting (FACS)

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Profiling of miRNA expression of HeLa cells infected with Salmonella Typhimurium

Project description:We identified miRNAs differentially regulated upon Salmonella infection by comparative deep-sequencing analysis of cDNA libraries prepared from the small RNA population (10–29 nt) of HeLa cells infected with Salmonella (20 hpi) and mock-treated cells. Considering that at a MOI of 25 Salmonella is internalized in only 10-15% of the HeLa cells, we separated the fraction of cells which had internalized Salmonella (Salmonella+) from the bystander fraction (Salmonella-) by fluorescence-activated cell sorting (FACS), and extended the analysis of miRNA changes to these samples. Interestingly, we observed that Salmonella infection induces a significant decrease in the expression of all the detected members of the miR-15 family

2014-07-18 | GSE53278 | GEO

Profiling of miRNA expression of HeLa cells infected with Salmonella Typhimurium

Project description:We identified miRNAs differentially regulated upon Salmonella infection by comparative deep-sequencing analysis of cDNA libraries prepared from the small RNA population (10M-bM-^@M-^S29 nt) of HeLa cells infected with Salmonella (20 hpi) and mock-treated cells. Considering that at a MOI of 25 Salmonella is internalized in only 10-15% of the HeLa cells, we separated the fraction of cells which had internalized Salmonella (Salmonella+) from the bystander fraction (Salmonella-) by fluorescence-activated cell sorting (FACS), and extended the analysis of miRNA changes to these samples. Interestingly, we observed that Salmonella infection induces a significant decrease in the expression of all the detected members of the miR-15 family miRNA profiles of HeLa cells infected with Salmonella Typhimurium were generated by deep sequencing, using Illumina HiSeq2000.

2014-07-18 | E-GEOD-53278 | biostudies-arrayexpress

SIK2-dependent Phosphoproteome and Interactome upon Salmonella infection

Project description:HeLa cells transfected with non-targeting control- and anti-SIK2 siRNA were left uninfected or infected with Salmonella and lysed 30 min pi. Following tryptic-digest peptides were labeled using TMT-pro 16-plex and phosphopeptides enriched. The endogenously tagged SIK2-HA IP workflow. Hela wild type or endogenous SIK2-HA-tagged HeLa cells were left uninfected or infected with Salmonella and lysed 1 h post-infection, followed by HA-IP, Tryptic digest, TMT-labeling and LC-MS/MS analysis.

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Project description:Transcriptomic profiling of HeLa cells infected with Shigella flexneri

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Transcriptomic profiling of HeLa cells infected with Salmonella Typhimurium

Project description:We evaluated the transcriptome changes induced by infection with Salmonella (20 hpi, MOI 100). Transcriptmic profiles of HeLa cells infected with Salmonella Typhimurium were generated by deep sequencing, using Illumina HiSeq 2000.

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Project description:Profiling of miRNA expression of HeLa cells infected with Salmonella Typhimurium

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