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Homo sapiens

ABSTRACT: Transcriptomic profiling of HeLa cells infected with Salmonella Typhimurium

OTHER RELATED OMICS DATASETS IN: E-GEOD-53280

PROVIDER: PRJNA231559 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1049363.fastq.gz	Fastqsanger.gz
	SRR1049364.fastq.gz	Fastqsanger.gz
	SRR1049365.fastq.gz	Fastqsanger.gz
	SRR1049366.fastq.gz	Fastqsanger.gz
	SRR1049367.fastq.gz	Fastqsanger.gz

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miRNA expression analysis of Salmonella Typhimurium infected cells

Project description:To have a global picture of the miRNAs regulated upon Salmonella infection, we assessed small RNA changes, by RNA-sequencing, of HeLa cells infected with Salmonella Typhimurium compared with mock-treated cells . In addtion to the total population, we evaluated miRNA expression in the fraction of HeLa cells with internalized bacteria (Salmonella-positive), as well as in bystander cells, separated by fluorescence activated cell sorting (FACS)

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Profiling of miRNA expression of HeLa cells infected with Salmonella Typhimurium

Project description:We identified miRNAs differentially regulated upon Salmonella infection by comparative deep-sequencing analysis of cDNA libraries prepared from the small RNA population (10M-bM-^@M-^S29 nt) of HeLa cells infected with Salmonella (20 hpi) and mock-treated cells. Considering that at a MOI of 25 Salmonella is internalized in only 10-15% of the HeLa cells, we separated the fraction of cells which had internalized Salmonella (Salmonella+) from the bystander fraction (Salmonella-) by fluorescence-activated cell sorting (FACS), and extended the analysis of miRNA changes to these samples. Interestingly, we observed that Salmonella infection induces a significant decrease in the expression of all the detected members of the miR-15 family miRNA profiles of HeLa cells infected with Salmonella Typhimurium were generated by deep sequencing, using Illumina HiSeq2000.

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