Project description:Transcriptional profiling of Arabidopsis thaliana seedlings treated with trans-2-hexenal, highlighting to the physiological function of plant volatile chemicals by observing early response of gene expressions in Arabidopsis seedlings.
Project description:Transcriptional profiling of Arabidopsis thaliana seedlings treated with trans-2-hexenal, highlighting to the physiological function of plant volatile chemicals by observing early response of gene expressions in Arabidopsis seedlings. Two-condition experiment, trans-2-hexenal-treated seedlings vs. control seedlings. Biological replicates:2 control replicates, 2 trans-2-hexenal-treated.
Project description:Plants are known to be responsive to volatiles, but knowledge about the molecular players involved in transducing their perception remain scarce. Here the response of Arabidopsis thaliana to E-2-hexenal, one of the green leaf volatiles that are produced upon wounding, herbivory or infection with pathogens is studied. We have taken a transcriptomics approach to identify genes that are induced by E-2-hexenal but not by defense hormones. In three independent biological experiments, Arabidopsis plants, ecotype Columbia (Col-0), were exposed either to 3 µM aerial E-2-hexenal or to the carrier MeOH for the mock treatment and rosette leaves were harvested after 1h, 3h and 24h.
Project description:Plants are known to be responsive to volatiles, but knowledge about the molecular players involved in transducing their perception remain scarce. Here the response of Arabidopsis thaliana to E-2-hexenal, one of the green leaf volatiles that are produced upon wounding, herbivory or infection with pathogens is studied. We have taken a transcriptomics approach to identify genes that are induced by E-2-hexenal but not by defense hormones.