Bos taurus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA241311 | ENA |
REPOSITORIES: ENA
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Project description:Mito-nuclear crosstalk underlying ovarian aging remains elusive. Single-cell transcriptomics of aged ovaries identified senescent signatures including dysregulated mitochondrial metabolism, disturbed histone modification and enriched SASP. We demonstrated that impaired SIRT5-mediated desuccinylation drives ovarian aging. As a major substrate of SIRT5 in the TCA cycle, SUCLG2 is regulated by desuccinylation at K93 and K101, which enhances its stability and activity to rescue mitochondrial dysfunction. Conversely, SUCLG2 hypersuccinylation leads to acetyl-CoA accumulation, increases nuclear H4K8ac and upregulates metabolic genes to compensate for energy deficiency. In vivo experiments showed excess acetyl-CoA accelerates ovarian aging, while a SUCLG2 desuccinylation mutant alleviates this defect. This study reveals the molecular basis of ovarian aging and highlights the SIRT5-SUCLG2 axis as a promising therapeutic target.
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Project description:Mito-nuclear crosstalk underlying ovarian aging remains elusive. Single-cell transcriptomics of aged ovaries identified senescent signatures including dysregulated mitochondrial metabolism, disturbed histone modification and enriched SASP. We demonstrated that impaired SIRT5-mediated desuccinylation drives ovarian aging. As a major substrate of SIRT5 in the TCA cycle, SUCLG2 is regulated by desuccinylation at K93 and K101, which enhances its stability and activity to rescue mitochondrial dysfunction. Conversely, SUCLG2 hypersuccinylation leads to acetyl-CoA accumulation, increases nuclear H4K8ac and upregulates metabolic genes to compensate for energy deficiency. In vivo experiments showed excess acetyl-CoA accelerates ovarian aging, while a SUCLG2 desuccinylation mutant alleviates this defect. This study reveals the molecular basis of ovarian aging and highlights the SIRT5-SUCLG2 axis as a promising therapeutic target.
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Project description:Coordinated interactions between ovarian granulosa and theca cells are required for female endocrine function and fertility. To elucidate these interactions the regulation of the granulosa and theca cell transcriptomes during bovine antral follicle development were investigated. Granulosa cells and theca cells were isolated from small (<5 mm), medium (5-10 mm), and large (>10 mm) antral bovine follicles. A microarray analysis of 24,000 bovine genes revealed that granulosa cells and theca cells each had gene sets specific to small, medium and large follicle cells. Transcripts regulated (i.e., minimally changed 1.5-fold) during antral follicle development for the granulosa cells involved 446 genes and for theca cells 248 genes. Only 28 regulated genes were common to both granulosa and theca cells. Regulated genes were functionally categorized with a focus on growth factors and cytokines expressed and regulated by the two cell types. Candidate regulatory growth factor proteins mediating both paracrine and autocrine cell-cell interactions include macrophage inflammatory protein (MIP1 beta), teratocarcinoma-derived growth factor 1 (TDGF1), stromal derived growth factor 1 (SDF1; i.e., CXCL12), growth differentiation factor 8 (GDF8), glia maturation factor gamma (GMFG), osteopontin (SPP1), angiopoietin 4 (ANGPT4), and chemokine ligands (CCL 2, 3, 5, and 8). The current study examined granulosa cell and theca cell regulated genes associated with bovine antral follicle development and identified candidate growth factors potentially involved in the regulation of cell-cell interactions required for ovarian function. Experiment Overall Design: Granulosacell RNA samples from three groups of follicles different in size - small, medium, and large (pooled untreated ovaries) are compared between each other. Each group has 2 separate biological replicas; each replica contained pooled RNA from 20-40 ovaries from 6-10 different animals.
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