Project description:We observed separation of COPD patients from control smokers by CD4+ T cell transcriptomics. In contrast to CD4+ T cells, there were no transcript differences in whole blood, CD8+ T cells or CD14+ monocytes.
Project description:We observed no transcripts in the whole blood that distinguishes COPD patients and control smokers. In contrast, T cell directed stimulation of whole blood, resulted in altered transcriptomic response and separated COPD patients from control smokers.
Project description:Differential profiles from whole genome human expression arrays on monocytes obtained from peripheral blood in COPD was studied and compared with controls. Monocytes were isolated from Controls (Group 1) which included Control Smokers (Group 1A) and Control Never Smokers (Group 1B) and COPD (Group 2) which included COPD Smokers (Group 2A) and COPD ExSmokers (Group 2B). Differential transcriptomic expression associated with (i) Smoking, (ii) COPD, and (iii) cessation of smoking were identified.
Project description:We generated transcriptomes of primary human lung AT2 cells isolated from distal parenchyma of ex-smoker controls and COPD patients, with both mild and severe disease.