Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA263018 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Primary and adjacent non-malignant tissue samples of 100 HCC patients (mRNA)
| PRJNA263020 | ENA
Project description:Genome-wide miRNA expression profiles of 100 primary and matched non-malignant tissues of HCC patients from a Singapore patient cohort. HCC is a hetergenous disease and it is important to understand the underlying molecular mechanisms. Here, we studied the role of microRNAs in HCC by integrating microRNA and gene expression profiles of HCC patients. Profiling of 100 primary and adjacent non-malignant HCC tissue samples on mirXplore two-color miRNA expression microarray. All tissues were collected with approvals from the National Cancer Centre, Singapore; the Research Ethics Review Committee; and signed patient informed consent.
2016-02-18 | E-GEOD-62007 | biostudies-arrayexpress
Project description:Genome-wide mRNA expression profiles of 100 primary and matched non-malignant tissues of HCC patients from a Singapore patient cohort. HCC is a hetergenous disease and it is important to understand the underlying molecular mechanisms. Here, we studied the role of microRNAs in HCC by integrating microRNA and gene expression profiles of HCC patients. Profiling of 100 primary and adjacent non-malignant HCC tissue samples on Agilent two-color whole human genome gene expression microarray (design G4112F). All tissues were collected with approvals from the National Cancer Centre, Singapore; the Research Ethics Review Committee; and signed patient informed consent.
2016-02-18 | E-GEOD-62043 | biostudies-arrayexpress
Project description:Genome-wide miRNA expression profiles of 100 primary and matched non-malignant tissues of HCC patients from a Singapore patient cohort. HCC is a hetergenous disease and it is important to understand the underlying molecular mechanisms. Here, we studied the role of microRNAs in HCC by integrating microRNA and gene expression profiles of HCC patients.
2016-02-18 | GSE62007 | GEO
Project description:Non-coding microRNAs (miRNAs) mainly regulate the expression of targeted genes by regulating mRNA degradation or repressing their protein translation. MiRNA microarray profiling was then performed on 218 human HCC tumors samples, 10 samples from adjacent cirrhotic non-tumoral tissue, 10 samples from healthy liver and 12 HCC cell lines. In this study we investigated which miRNAs were differentially expressed in HCC compared to cirrhotic non-tumoral tissue and healthy liver.
2016-11-03 | GSE74618 | GEO
Project description:Genome-wide mRNA expression profiles of 100 primary and matched non-malignant tissues of HCC patients from a Singapore patient cohort. HCC is a hetergenous disease and it is important to understand the underlying molecular mechanisms. Here, we studied the role of microRNAs in HCC by integrating microRNA and gene expression profiles of HCC patients.
2016-02-18 | GSE62043 | GEO
Project description:microRNA microarray expression profiling comparing tumor versus paired adjacent non-tumor from 100 HCC patients
2016-12-31 | GSE28854 | GEO
Project description:To study its expression and regulatory functions in hepatocellular carcinoma (HCC) progression, whole transcriptome RNA sequencing in paired HCC tumor and adjacent non-tumor liver tissue samples from 3 local Hispanic patients was performed.
2024-09-25 | GSE233421 | GEO
Project description:Dysregulation of ubiquitin-proteasome pathway genes through copy number alteration, promoter hypomethylation, and miRNA deregulation is involved in cancer development and progression. Further characterizing alterations in these genes may uncover novel drug targets across a range of diseases in which druggable alterations are uncommon, including hepatocellular carcinoma (HCC). We analyzed 377 HCC and 59 adjacent non-malignant liver tissue samples, focusing on alterations to component genes of the widely studied CRL2pVHL complex. Patient outcome was significantly worse when complex components VHL, CUL2, ELOC, or RBX1 were overexpressed in tumours. DNA hypomethylation and copy number increase were correlated with this overexpression, but many tumours had mRNA upregulation that was not explained by either mechanism. To assess the potential role of miRNA downregulation in component gene upregulation, we first acquired a wide-ranging view of the liver miRNA transcriptome by finding all miRNAs that were expressed in fetal liver, HCC, or adjacent non-malignant samples. Interestingly, we found 66 miRNAs, including 39 previously unannotated miRNAs, that were downregulated in HCC and predicted to target one or more CRL2pVHL components. Several miRNAs, including hsa-miR-101-3p and hsa-miR-139-5p, were negatively correlated with multiple component genes, suggesting that miRNA deregulation may contribute to CRL2pVHL overexpression. Combining miRNA and mRNA expression, DNA copy number, and methylation status into one multidimensional survival analysis, we found a significant association between greater numbers of alterations and poorer overall survival for multiple component genes. While the intricacies of CRL2pVHL complex gene regulation require additional research, it is evident that multiple causes for the deregulation of these genes must be considered in HCC, including non-traditional mechanisms. A better understanding of the CRL2pVHL components and further elucidation of their regulation could expand our understanding of HCC oncogenesis and aid in the development of novel inhibitors.
2022-06-21 | GSE194052 | GEO