ABSTRACT: Differential gene transcript expression profiles among human monocyte-derived dendritic cells infected with Leishmania major Friedlin V1 strain and Leishmania donovani 1S strain parasites at early infection time points
Project description:Monocyte derived dendritic cells (MDDC) were infected with Leishmania major or Leishmania donovani parasites and collected at 4, 8, and 24 hours post-infection to analyze the differential effects those parasite species have on human host cell gene expression over time. Monocyte derived dendritic cells (MDDC) were generated from blood buffy coats collected from five anonymous healthy human donors and infected 10:1 (parasite to host cell) with Leishmania major Friedlin V1 strain or Leishmania donovani 1S strain parasites, where after 4, 8, or 24 hours total RNA was harvested from cells, cDNA generated, and hybridized to human gene transcipt expression arrays to assess differential host cell gene transcriptional expression differences relative to uninfected cells.
Project description:Monocyte derived dendritic cells (MDDC) were infected with Leishmania major parasites which were knockout mutants of either lpg1 or lpg2 genes that responsible for expression of surface molecular structures on the parasites in order to assess the effects those molecules have on human host cell gene expression. Monocyte derived dendritic cells (MDDC) were generated from blood buffy coats collected from four anonymous healthy human donors and infected 10:1 (parasite to host cell) with Leishmania major Friedlin V1 strain wildtype parasites and lipophosphogylcan mutants, where after total RNA was harvested, cDNA generated, and hybridized to human gene transcipt expression arrays to assess differential host cell gene transcriptional expression differences relative to uninfected cells. Please note that the final RMA normalized background value for each array was provided in the characteristics field and any data that was below the background value was filtered out.
Project description:Monocyte derived dendritic cells (MDDC) were infected with Leishmania major or Leishmania donovani parasites and collected at 4, 8, and 24 hours post-infection to analyze the differential effects those parasite species have on human host cell gene expression over time.
Project description:Monocyte-derived dendritic cells (DC) and macrophages (MΦ) generated in vitro from the same individual blood donors were exposed to five different pathogens, and gene expression profiles were assessed by microarray analysis. Responses to Mycobacterium tuberculosis and to phylogenetically distinct protozoan (Leishmania major, L. donovani, Toxoplasma gondii) and helminth (Brugia malayi) parasites were examined, each of which produces chronic infections in humans yet vary considerably in the nature of the immune responses they trigger. Keywords: equivalent probe
Project description:Monocyte-derived dendritic cells (DC) and macrophages (MΦ) generated in vitro from the same individual blood donors were exposed to five different pathogens, and gene expression profiles were assessed by microarray analysis. Responses to Mycobacterium tuberculosis and to phylogenetically distinct protozoan (Leishmania major, L. donovani, Toxoplasma gondii) and helminth (Brugia malayi) parasites were examined, each of which produces chronic infections in humans yet vary considerably in the nature of the immune responses they trigger.<br><br>Note that the following pairs of files as imported from GEO are identical: GSM5135.txt and GSM5144.txt; GSM5137.txt and GSM5138.txt; GSM5143.txt and GSM5150.txt; GSM5148.txt and GSM5155.txt
Project description:Monocyte derived dendritic cells (MDDC) were infected with Leishmania major parasites which were knockout mutants of either lpg1 or lpg2 genes that responsible for expression of surface molecular structures on the parasites in order to assess the effects those molecules have on human host cell gene expression.
