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Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage

ABSTRACT: Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage

PROVIDER: PRJNA272312 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1751360.fastq.gz	Fastqsanger.gz
	SRR1751370.fastq.gz	Fastqsanger.gz
	SRR1751361.fastq.gz	Fastqsanger.gz
	SRR1751371.fastq.gz	Fastqsanger.gz
	SRR1751362.fastq.gz	Fastqsanger.gz

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Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [RNA-seq]

Project description:MyoD and NeuroD2 are master regulators of myogenesis and neurogenesis and bind to a "shared" E-box sequence (CAGCTG) and a "private" sequence (CAGGTG or CAGATG, respectively). To determine whether private-site recognition is sufficient to confer lineage-specification, we generated a MyoD-mutant with the DNA binding specificity of NeuroD2. Our results demonstrate that redirecting MyoD binding from MyoD-private sites to NeuroD2-private sites, despite preserved binding to the MyoD/NeuroD2-shared sites, is sufficient to change MyoD from a master regulator of myogenesis to a master regulator of neurogenesis. RNA-seq profiling of mouse P19 cells transfected with MyoD, NeuroD2 and chimera mutants. The chimeric mutants are MyoD with the bHLH domain replaced with the NeuroD2 bHLH domain.

2015-07-31 | E-GEOD-64622 | biostudies-arrayexpress

Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [ChIP-seq]

Project description:MyoD and NeuroD2 are master regulators of myogenesis and neurogenesis and bind to a "shared" E-box sequence (CAGCTG) and a "private" sequence (CAGGTG or CAGATG, respectively). To determine whether private-site recognition is sufficient to confer lineage-specification, we generated a MyoD-mutant with the DNA binding specificity of NeuroD2. Our results demonstrate that redirecting MyoD binding from MyoD-private sites to NeuroD2-private sites, despite preserved binding to the MyoD/NeuroD2-shared sites, is sufficient to change MyoD from a master regulator of myogenesis to a master regulator of neurogenesis. ChIP-seq profiling of MyoD, NeuroD2 and chimera mutants in mouse P19 cells transfected with these genes. The chimeric mutants are MyoD with the bHLH domain replaced with the NeuroD2 bHLH domain.

2015-07-31 | E-GEOD-64626 | biostudies-arrayexpress

Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [ChIP-seq]

2015-07-31 | GSE64626 | GEO

Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [RNA-seq]

2015-07-31 | GSE64622 | GEO

Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage.

Project description:MyoD and NeuroD2, master regulators of myogenesis and neurogenesis, bind to a "shared" E-box sequence (CAGCTG) and a "private" sequence (CAGGTG or CAGATG, respectively). To determine whether private-site recognition is sufficient to confer lineage specification, we generated a MyoD mutant with the DNA-binding specificity of NeuroD2. This chimeric mutant gained binding to NeuroD2 private sites but maintained binding to a subset of MyoD-specific sites, activating part of both the muscle and neuronal programs. Sequence analysis revealed an enrichment for PBX/MEIS motifs at the subset of MyoD-specific sites bound by the chimera, and point mutations that prevent MyoD interaction with PBX/MEIS converted the chimera to a pure neurogenic factor. Therefore, redirecting MyoD binding from MyoD private sites to NeuroD2 private sites, despite preserved binding to the MyoD/NeuroD2 shared sites, is sufficient to change MyoD from a master regulator of myogenesis to a master regulator of neurogenesis.

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Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-07-31 | E-GEOD-64627 | biostudies-arrayexpress

Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-07-31 | GSE64627 | GEO

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Project description:Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [RNA-seq]

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Mus musculus

Project description:Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [ChIP-seq]

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MYOD Gene Expression Regulation during Myogenic Conversion of Fibroblasts

Project description:We provide here gene expression profile of IMR90 fibroblasts expressing or not MYOD culured in GM for 1 day or GM for 1 day and DM for 3 days

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