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No evidence for a C. elegans small RNA response to feeding with OxyS-expressing bacteria

ABSTRACT: No evidence for a C. elegans small RNA response to feeding with OxyS-expressing bacteria

PROVIDER: PRJNA272634 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1759870.fastq.gz	Fastqsanger.gz
	SRR1759871.fastq.gz	Fastqsanger.gz
	SRR1759872.fastq.gz	Fastqsanger.gz
	SRR1759864.fastq.gz	Fastqsanger.gz
	SRR1759865.fastq.gz	Fastqsanger.gz

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No evidence for a C. elegans small RNA response to feeding with OxyS-expressing bacteria

Project description:Small RNA sequencing shows that there are no Caenorhabditis elegans endo siRNAs made against the OxyS non-coding RNA.

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No evidence for a C. elegans small RNA response to feeding with OxyS-expressing bacteria

Project description:Small RNA sequencing shows that there are no Caenorhabditis elegans endo siRNAs made against the OxyS non-coding RNA. Sequencing of small RNAs from C. elegans fed bacteria overexpressing the non-coding RNA OxyS, control samples fed either standard E. coli or one without the OxyS RNA, an rde-4 deletion mutant deficient in endo siRNA production and the OxyS-overexpressing bacteria itself. 5' independent samples were treated with the enzyme RNA 5' polyphosphatase to remove 5' triphosphate; 5' dependent samples were not treated with 5' polyphosphatase.

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C. elegans small RNA sequences from wild type animals fed on sel-1 dsRNA producing bacteria

Project description:RNA interference (RNAi) is a phylogenetically widespread gene silencing process triggered by doublestranded RNA (dsRNA). In plants and C. elegans, two distinct populations of small RNAs have been proposed to participate in RNAi : "Primary siRNAs" (derived from Dicer nuclease-mediated cleavage of the original trigger) and "Secondary siRNAs" (additional small RNAs whose synthesis requires an RNA-directed RNA polymerase [RdRP]). Analyzing small RNAs associated with ongoing RNAi in C. elegans, we found secondary siRNAs to comprise the vast majority. The bulk of secondary siRNAs exhibited structure and sequence indicative of a biosynthetic mode where each molecule derives from an independent de novo initiation by RdRP. Analysis of endogenous small RNAs indicated that a fraction derive from a biosynthetic mechanism that is similar to that of secondary siRNAs formed during RNAi, suggesting that small antisense transcripts derived from cellular mRNAs by RdRP activity may have key roles in cellular regulation. Keywords: C. elegans small RNA sequences from wild type animals fed on sel-1 dsRNA producing bacteria

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