Project description:We compared gene expression between high fat diet (HFD)-fed Adipo-NDUFS4 knockout (KO) and WT mice in inguinal white adipose tissue (iWAT or scWAT).
Project description:Differentiation of brown adipocytes is a crucial process for adaptive thermogenesis, which is stimulated by various factors. We found robust browning of inguinal white adipose tissue in UCP1/ApoE-DKO mice, but not in ApoE-KO mice, under high-fat diet condition. We used microarray to determine the genes specifically regulated in the browning white adipose tissue in UCP1/ApoE-DKO mice.
Project description:Using high throughput sequencing we report 5hmC levels, and gene expression changes in mouse Inguinal white adipose tissue upon exposure to room temperature and 7 days to cold in Tet1 floxed wild-type control and adipose selectiveTet1 KO mice.
Project description:We analyzed transcript abundance in interscapular brown and inguinal white adipose tissue of wildtype and UCP1-KO mice either adpated to 20°C or 30°C and fed a high fat or control diet.
Project description:Cold stimulation not only activates the thermogenesis activity of brown adipose tissue (BAT) but also induces browning of white adipose tissue (WAT). To elucidate the mechanisms underlying cold-induced thermogenesis and adipose tissue remodeling, we used RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to examine the transcriptomic and proteomic profiles, respectively, of adipose tissue from mice exposed to cold or thermoneutral temperature. The male C57BL/6J mice were divided into three groups (5 mice/group), two groups were kept at 6 ˚C for 6 h and 24 h, respectively, and the control group were kept at 22 ˚C for 24 h. Subsequently, the BAT and inguinal WAT of each mouse were dissected and subjected to RNA-seq and data-independent acquisition (DIA)-based LC-MS/MS.
Project description:Adipokine Adissp simultaneously promotes glucose disposal and energy expenditure in adipose tissue. Treatment with recombinant Adissp (rAdissp) protein normalizes hyperglycemia and provides pleiotropic cardiometabolic benefits in diabetic and obese mice, primarily through its actions in white adipose tissue. RNA-seq analysis of inguinal white fat reveals that rAdissp activates a comprehensive thermogenic program characterized by enhanced mitochondrial metabolism, Ucp1-mediated uncoupling, glycolysis, and fatty acid futile cycling.
