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Proteomic characterization of adipose tissue from cold-exposed mice

ABSTRACT: Cold stimulation not only activates the thermogenesis activity of brown adipose tissue (BAT) but also induces browning of white adipose tissue (WAT). To elucidate the mechanisms underlying cold-induced thermogenesis and adipose tissue remodeling, we used RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to examine the transcriptomic and proteomic profiles, respectively, of adipose tissue from mice exposed to cold or thermoneutral temperature. The male C57BL/6J mice were divided into three groups (5 mice/group), two groups were kept at 6 ˚C for 6 h and 24 h, respectively, and the control group were kept at 22 ˚C for 24 h. Subsequently, the BAT and inguinal WAT of each mouse were dissected and subjected to RNA-seq and data-independent acquisition (DIA)-based LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Adipose Tissue

SUBMITTER: Xing Zhang

LAB HEAD: Xing Zhang

PROVIDER: PXD067973 | Pride | 2026-01-09

REPOSITORIES: Pride

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