Project description:Sialyllactose supplementation in pig milk replacer could promote cognitive functions in postnatal piglet behaviour experiment. There are 2 groups of 3-day-old postnatal piglets, feeding basal level and sialyllactose. After 35 days feeding, sialyllactose group showed better performance in behaviour experiment. We used microarrays to detail the global programme of gene expression underlying the nervous system development and memory formation.
Project description:The four-eyed fish Anableps anableps exhibits a unique visual system adapted for simultaneous aerial and aquatic vision, making it an exceptional model for investigating evolutionary innovations in vertebrate eye development. This dual vision capability is supported by specialized adaptations to the eye, including a thicker dorsal (aerial) cornea and asymmetric expression of visual opsins: opn1mw1 in the ventral retina, and opn1lw1 in the dorsal retina. To investigate the molecular and spatial basis of this asymmetry, we used single-nucleus RNA sequencing (snRNAseq) of the retina in combination with spatial transcriptomics of the whole eye. Our findings reveal that the asymmetric opsin distribution results from a developmental expansion of the ventral retina, which is adapted for detecting aerial light, alongside a reduction in the dorsal retinal domain responsible for aquatic vision. These results highlight a novel mechanism of regional specialization in the vertebrate retina and provide insights into the evolutionary innovations in the vertebrate visual system.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
