Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA278074 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:To further elucidate the gene expression profile alterations induced by HCV infection, we have employed the Arraystar Human LncRNA Microarray V3.0 as a discovery platform to identify genes associated with HCV infection. Human hepatocellular carcinoma cell line Huh7.5.1 was infected with HCVcc for 6 h. The gene expression pattern of HCVcc-infected Huh7.5.1 was compared with that of uninfected Huh7.5.1 to identify the differentially expressed genes induced by HCV infection.
2023-11-21 | GSE233543 | GEO
Project description:Cytosolic lipid droplets (LDs) are vital to Hepatitis C Virus (HCV) infection as the putative sites of virion assembly. To identify novel regulators of HCV particle production, we performed quantitative LD proteome analysis. Huh7.5 cells were labeled by stable isotope labeling with heavy amino acids in cell culture (SILAC) and subsequently infected with an HCV Jc1 reporter virus. After selection for HCV-infected cells, equal amounts of HCV-infected and uninfected control cells were mixed, LDs were isolated and analyzed by LC-ESI-MS/MS.
2016-09-26 | PXD004707 | Pride
Project description:Time series data of HCV (JC1) infection of Huh7 cells
| PRJNA168577 | ENA
Project description:Huh7/5-2 cells (Binder et al., Hepatology 2007) were mock infected (DMEM) (time points 4 and 48 h) or infected with the chimeric HCV virus Jc1 (Pietschmann et al., PNAS 2006) (all time points). Multiplicity of infection was 15 (TCID50). Cells were lysed after 4, 12, 24, 48 and 72 hours post infection and total cellular RNA was prepared.
2012-06-15 | GSE38720 | GEO
Project description:Prognostic Liver Signature profiles of Jc1-infected Huh7.5.1dif cells treated with various drugs
| PRJNA322559 | ENA
Project description:Transcription profiling by array of human hepatoma Huh7.5.1-derived cell clones; Huh7.5.1-8 cells highly permissive to HCV and S7-A cells resistant to HCV.
2014-12-31 | E-MTAB-2589 | biostudies-arrayexpress
Project description:Endoribonuclease activity in hepatitis C virus-infected Huh7.5.1 cells
| PRJNA293203 | ENA
Project description:Huh7/5-2 cells (Binder et al., Hepatology 2007) were mock infected (DMEM) (time points 4 and 48 h) or infected with the chimeric HCV virus Jc1 (Pietschmann et al., PNAS 2006) (all time points). Multiplicity of infection was 15 (TCID50). Cells were lysed after 4, 12, 24, 48 and 72 hours post infection and total cellular RNA was prepared. Mock infected cells serve as controls for the infected samples. The whole experiment was repeated on independent days (2 biological replicates). The dataset was also submitted to GEO (GSE38720).
2012-06-30 | E-MTAB-1148 | biostudies-arrayexpress
Project description:Chronic hepatitis C virus (HCV) infection is a leading cause of liver cancer. HCV propagation and oncogenicity depend in part on the phosphorylation states of its non-structural protein 5A (NS5A); however, little is known about how hypo- or hyper-phosphorylated NS5A functions. Here, we segregated hypo- from hyper-phosphorylated NS5A in HCV-infected Huh7.5.1 cells with two custom-made specific antibodies and differentiated their interacting proteins with dimethyl labeling-based quantitative proteomics. Bioinformatics analysis revealed that hyper-phosphorylated NS5A preferentially binds the polymerase II-associated factor 1 complex known to alter host gene expression involved in cancer progression. In contrast, hypo-phosphorylated NS5A binds proteins involved in host antiviral response. Moreover, we found that the hypo-phosphorylated NS5A binds DNA-dependent protein kinase catalytic subunit (DNA-PKcs) predicted to phosphorylate NS5A at serine 232, a key amino acid that governs NS5A transition from hypo- to hyper-phosphorylation state. Inhibition of DNA-PKcs with an inhibitor or via gene-specific knockdown significantly reduced serine 232 phosphorylation and NS5A hyper-phosphorylation. Collectively, we have identified a protein kinase that regulates a delicate balance of NS5A between hypo- and hyper-phosphorylation states respectively involved in host antiviral responses and liver cancer progression.
2019-06-17 | PXD012767 | Pride
Project description:Host transcriptomic response to HCV genotype 2a (JFH1) infection in Huh7.5.1 cells
| PRJNA1261973 | ENA