Project description:Emerging data has highlighted the importance of short-chain fatty acids (SCFAs) on ruminal microbiome and derived metabolism profiling, and ruminal epithelial health and nutritional absorption in ruminants. However, little is known about the roles of SCFAs on ileal microbiome profiles. Here, we combined infusion of three SCFAs, to study their different roles in ileal microbiome succession profiling using a in vivo goat model.
Project description:Emerging data has highlighted the importance of short-chain fatty acids (SCFAs) on ruminal microbiome and derived metabolism profiling, and ruminal epithelial health and nutritional absorption in ruminants. However, little is known about the roles of SCFAs on hindgut profiles. Here, we firstly combined infusion of three SCFAs, to study their different roles in hindgut microbiome succession and derived metabolism profiling, as well as colonic epithelial transcriptome sequencing patterns using a in vivo goat model. .
Project description:Emerging data has highlighted the importance of short-chain fatty acids (SCFAs) on ruminal microbiome and derived metabolism profiling, and ruminal epithelial health and nutritional absorption in ruminants. However, little is known about the roles of SCFAs on hindgut profiles. Here, we firstly combined infusion of three SCFAs, to study their different roles in hindgut microbiome succession and derived metabolism profiling, as well as colonic epithelial transcriptome sequencing patterns using a in vivo goat model.
Project description:Short-chain fatty acids (SCFAs) are microbial metabolites, also known as postbiotics, produced by the gut microbiotame, playing essential rolesessential in maintaining gut health and exerting potential anticancer effects. This study investigates the antiproliferative effects of short-chain fatty acids (SCFAs) salts—magnesium acetate (A), sodium propionate (P), and sodium butyrate (B)—and their combinations with dexamethasone (Dex) in AGS gastric adenocarcinoma cells.
Project description:Short-chain fatty acids (SCFAs) are microbial metabolites, also known as postbiotics, produced by the gut microbiotame, playing essential rolesessential in maintaining gut health and exerting potential anticancer effects. This study investigates the antiproliferative effects of short-chain fatty acids (SCFAs) salts—magnesium acetate (A), sodium propionate (P), and sodium butyrate (B)—and their combinations with doxorubicin (Dox) in AGS gastric adenocarcinoma cells.
Project description:Acetate, propionate and butyrate are the main short-chain fatty acids (SCFAs) that arise from the fermentation of fibers by the colonic microbiota. While many studies focus on the regulatory role of SCFAs, their quantitative role as a catabolic or anabolic substrate for the host has received relatively little attention. To investigate this aspect, we infused conscious mice with physiological quantities of stable isotopes [1-13C]acetate, [2-13C]propionate or [2,4-13C2]butyrate directly into the cecum, which is the natural production site in mice, and analyzed their interconversion by the microbiota as well as their metabolism by the host. Cecal interconversion - pointing to microbial cross-feeding - was high between acetate and butyrate, low between butyrate and propionate and almost absent between acetate and propionate. As much as 62% of infused propionate was used in whole-body glucose production, in line with its role as gluconeogenic substrate. Conversely, glucose synthesis from propionate accounted for 69% of total glucose production. The synthesis of palmitate and cholesterol in the liver was high from cecal acetate (2.8% and 0.7%, respectively) and butyrate (2.7% and 0.9%, respectively) as substrates, but low or absent from propionate (0.6% and 0.0%, respectively). Label incorporation due to chain elongation of stearate was approximately 8-fold higher than de novo synthesis of stearate. Microarray data suggested that SCFAs exert only a mild regulatory effect on the expression of genes involved in hepatic metabolic pathways during the 6h infusion period. Altogether, gut-derived acetate, propionate and butyrate play important roles as substrates for glucose, cholesterol and lipid metabolism. Mice were infused in cecum with stably-labelled isotopes of the three main short chain fatty acids or control solution. After 6 hrs, livers were removed and pooled RNA samples were subjected to gene expression profiling.
Project description:This is to determine the regulation of gene expression in different T Cell population with short chain fatty acids. This will provide the roles of SCFAs in regulation of adaptive immunity and T-cell-mediated inflammation.
Project description:Emerging data has highlighted the importance of short-chain fatty acids (SCFAs), particularly butyrate, in regulating ruminal homeostasis in vivo isolated epithelial cells. However, little is known about other SCFAs like acetate or propionate, and the interaction between rumen microbes and epithelial immunity are rarely reported. Here, we firstly combined infusion of three SCFAs, to study their different roles in ruminal development, antioxidant capacity, barrier functions, and immunity, as well as cross-talk with ruminal microbiome (16S rRNA sequencing data of rumen digesta) and derived transcriptome (RNA-Seq) and metabolism using an in vivo goat model.
Project description:Emerging data has highlighted the importance of short-chain fatty acids (SCFAs), particularly butyrate, in regulating ruminal homeostasis in vivo isolated epithelial cells. However, little is known about other SCFAs like acetate or propionate, and the interaction between rumen microbes and epithelial immunity are rarely reported. Here, we firstly combined infusion of three SCFAs, to study their different roles in ruminal development, antioxidant capacity, barrier functions, and immunity, as well as cross-talk with ruminal microbiome (16S rRNA sequencing data of rumen digesta) and derived transcriptome (RNA-Seq) and metabolism using an in vivo goat model.
Project description:Secondary metabolites such as short chain fatty acids (SCFAs) are primarily responsible for skin microbiota to modulate skin homeostasis. We here report propionic acid can relieve AD in mice. To further explore the mechanism underlying the modulation of skin microbiota-derived propionic acid on AD, we performed RNA-seq analysis on the skin lesions of ctrl or propionic acid-treated MC903 induced AD mice.
