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Mus musculus

ABSTRACT: Transcriptome analysis of early differentiating Tfh vs Th1 cells

PROVIDER: PRJNA279531 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1942820.fastq.gz	Fastqsanger.gz
	SRR1942830.fastq.gz	Fastqsanger.gz
	SRR1942821.fastq.gz	Fastqsanger.gz
	SRR1942822.fastq.gz	Fastqsanger.gz
	SRR1942823.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of early differentiating Tfh vs Th1 cells

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Transcriptome analysis of early differentiating Tfh vs Th1 cells

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Project description:The goal of this study was to define the regulation of Tfh cell response during intestinal helminth infection. Using the helminth Trichuris muris, we performed RNA-seq analysis on Tfh cells that develop during acute vs chronic helminth infection. We found that the transcriptomic profile of acute vs chronic induced Tfh cells are distinct and they express Th2/Th1-associated genes, respectively.

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Project description:We did microarray experiments to elucidate the transcriptome changes due to TCF-1 deficiency in Tfh cells. These data clearly demonstrate that Tfh-associated genes were down-regulated in TCF-1-null Tfh cells, whereas Th1-associated signatures were up-regulated, indicating that TCF-1 initiates the Tfh fate by directly strengthening Tfh signatures while suppressing Th1-associated genes. We first sorted WT and TCF-1-null Tfh cells (2 samples per group, each sample was pooled from 4 mice) at day 8 after LCMV Armstrong infection. Then, we extracted RNA, which were subsequently sent to microarray analysis.

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