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Mus musculus

ABSTRACT: Deep sequencing identifies PD0325901 modulated microRNAs in the J1 mESCs

PROVIDER: PRJNA280279 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1952004.fastq.gz	Fastqsanger.gz
	SRR1952005.fastq.gz	Fastqsanger.gz

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Project description:Deep sequencing identifies small molecule modulated microRNAs in the J1 mESCs

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Deep sequencing identifies PD0325901 modulated microRNAs in the J1 mESCs

Project description:PD0325901 is involved in improving reprogramming efficiency during inducing pluripotent stem cells (iPSC) or maintain a blastocyst-like state in embryonic stem cells (ESC). However, the knowledge about this small molecule regulating miRNAs in ESC was limited. To understand the role of miRNAs during PD03-induced ESC maintenance and gain an insight how PD0325901 regulates miRNAs expression; we performed small RNA sequencing using Illumina HiSeq 2000 under the compounds treatment. The data show the miRNAs regulated by PD0325901. J1 mESCs maintained in medium containing 1000 U/mL LIF and supplemented with PD0325901 for 24 hours, J1 treated with DMSO was set as control. Then total RNA was extracted for analysis.

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Identifying PD0325901-regulated genes in the J1 mESCs

Project description:It has been demonstrated that PD0325901 promotes self-renewal of mouse embryonic stem cells, however, the target genes of PD0325901 are not fully understood. To better understand the downstream target genes of PD0325901, we performed Microarray analyses to identify their downstream targets. The data show the genes regulated by PD0325901. J1 mESCs maintained in medium containing 1000 U/mL LIF and supplemented without or with PD0325901 for 24 hours, then total RNA was extracted for analysis.

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