Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA283136 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Kidney transplantation is the treatment of choice for patients with end-stage chronic kidney disease (ESKD). Despite the usefulness of transplantation as replacement therapy, long-term graft survival represents a major challenge for transplant immunology. Although nowadays there has been an advance in understanding immunological mechanisms mediating rejection, and the improvement of immunomodulation therapies, there are still underlying molecular processes marking an important variability among patients, and presumably influencing allograft rejection. With our analysis we explored differences in gene expression by Next Generation Sequencing implementing RNA-Seq in biopsies, blood and urine from kidney transplant patients with acute and chronic rejection. For this, we performed an intra-outcome analysis simultaneously in acute and chronic rejection, with which we sought: 1. To identify differences in gene expression between peripheral blood vs renal tissue and peripheral blood vs urine in acute rejection and chronic rejection; 2. To identify the level of agreement in gene expression between renal tissue and urine in acute rejection and chronic rejection and 3. To identify genes and biological processes associated with acute rejection and chronic rejection that could be potentially detected in blood, and simultaneously in urine and biopsy in acute rejection and in chronic rejection.
2023-12-31 | GSE242302 | GEO
Project description:Asthma is a chronic inflammatory airway disease characterized by airway inflammation and remodeling. The role of 15-oxo-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-oxoETE), a 15-HETE metabolite catalyzed by 15-prostaglandin dehydrogenase (15-PGDH), has been relatively unexplored in asthma. In this study, we used RNA-seq to explore the effect of 15-KETE on the transcriptome of airway epithelial cells, aiming to identify its potential downstream targets and mechanisms of action.
2026-05-21 | GSE325003 | GEO
Project description:The post kidney transplant period is associated with insulin resistance partly due to corticosteroids. Patients on minimal corticosteroid therapy receiving tacrolimus (FK506) for immunosuppression also are affected. FK506 inhibits calcineurin in immune cells. Skeletal muscle calcineurin, which also might be inhibited, mediates exercise-induced transcription leading to more insulin-sensitive muscle. This study was performed to determine if gene transcription responses to exercise are lower in patients awaiting kidney transplant or 3 months post-transplant on a low-steroid, FK506 protocol.
2015-09-30 | GSE68585 | GEO
Project description:Treatment with angiotensin converting enzyme inhibitors (ACEI) or angiotensin receptor blockers (ARB) has been shown to have anti-inflammatory effects such as decreased growth factors and cytokines in animal models, this effect however, has not been investigated in kidney transplant recipients. We aimed to study the effect of ACEI or ARB treatment on intragraft gene expression profiles of transplant kidney biopsies using microarrays. Both groups had similar demographic characteristics in terms of age, race, sex, type of transplant, previous history of transplantation or acute rejection, panel reactive antibody levels, and immunosuppressive treatment. There were no differences in acute and chronic Banff allograft injury scores between the 2 Groups. Intragraft gene expression profiles of ACEI or ARB treated Group 2 biopsies showed decreased gene transcripts of interferon-gamma and rejection-associated transcripts (GRIT) and constitutive macrophage-associated transcripts (CMAT) compared to Group 1 biopsies. There were no statistically significant differences in expression of cytotoxic T cell (CAT), regulatory T cell (TREG), B-cell (BAT), natural killer cell (NKAT), or endothelial cell-associated transcripts (ENDAT) between the 2 Groups. Our data suggest that exposure to ACEI or ARB was associated with down-regulation of GRIT and CMAT. This anti-inflammatory effect of ACEI or ARB treatment could be an additional benefit in kidney transplant recipients. We identified 29 near normal biopsies with chronic sum allograft injury score (ct+ci+cv) ⤠3 for gene expression profiling comparing 2 groups; Group 1 (n=16), patients with no exposure of ACEI or ARB treatment and Group 2 patients (n=13) with exposure to ACEI or ARB at least 6 months prior to kidney biopsy. Biopsies with a diagnosis of acute or chronic rejection, recurrent or de novo glomerular disease, or polyoma nephropathy were excluded.
2016-06-10 | E-GEOD-83095 | biostudies-arrayexpress
Project description:Chronic kidney disease (CKD) and end-stage renal disease (ESRD) are serious health concerns worldwide that require regular dialysis before a kidney transplant for a better quality of life. Due to aging and frailty, kidney dialysis patients are often limited in physical activity, which can lead to multiple health complications and sometimes result in patient death. Therefore, maintaining muscle health is crucial for kidney dialysis. We recently reported that personalized resistance-based exercise can improve frailty in kidney dialysis patients; however, the underlying molecular mechanisms by which resistance-based exercise improves muscle health are unknown. RNA-Seq analysis revealed that exercise promotes the expression of genes related to muscle stemness, regeneration, and differentiation. The IHC analysis revealed increased protein expression of stemness-associated proteins, including Lamin A/C, 5'-adenosine monophosphate-activated protein kinase (AMPK), paired box 7 (PAX7), vascular cell adhesion molecule 1 (VCAM-1), and Emerin. The IF analysis revealed increased protein expression of muscle differentiation markers, including myoblast determination protein 1 (MyoD), myosin heavy chain (MyoH), myogenin, desmin, dystrophin, and enolase 3. Furthermore, our study demonstrated that exercise upregulated proteins such as mitochondrial encoded cytochrome c oxidase III (MTCO3) and aconitase 2 (ACO2), which are involved in the oxidation of organic compounds and mitochondrial function in muscle. Moreover, we observed that exercise increased the protein expression of glucose transporter protein types 1 and 4 (GLUT1 and GLUT-4), glycogen synthase 1 and 2 (GYS1 and GYS2), and fatty acid binding protein 1 (FABP1), which participate in energy production through glucose uptake, glycogenesis, and fatty acid metabolism, respectively. These results suggest that personalized resistance-based muscle therapy in dialysis patients promotes the expression of genes and proteins associated with muscle stemness, regeneration, differentiation, and energy generation.
2026-03-18 | GSE309619 | GEO
Project description:Whole blood gene expression analysis of stable and acute rejection pediatric kidney transplant patients
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Project description:Mitochondrial dysregulation and oxidative stress in patients with chronic kidney disease
| PRJNA114765 | ENA
Project description:Plasma transcriptome profiling in patients with chronic kidney disease and end-stage renal disease
| PRJNA382706 | ENA
Project description:Peripheral blood biomarker signatures for acute kidney transplant rejection
| PRJNA116589 | ENA