Project description:Transcriptional profiling of human monocyte-derived dendritic cells, comparing cells transfected for 24h with Pre-miR-650 or nonsense control. 2 condition experiment: nonsense control versus miR-650 mimic - 3 biological replicates for each condition and control
Project description:Transcriptional profiling of human monocyte-derived dendritic cells, comparing cells transfected for 24h with Pre-miR-650 or nonsense control.
Project description:Transcriptional profiling of human monocyte-derived dendritic cells (MDDCs), comparing cells transfected with miR-29a mimic, or miR-29b mimic, or negative control.
Project description:Transcriptional profiling of human monocyte-derived dendritic cells (MDDCs), comparing cells transfected with miR-29a mimic, or miR-29b mimic, or negative control. 3 condition experiment, control versus miR-29a, and control versus miR-29b. 3 biological replicates for each condition and control.
Project description:As supplies of monocytes, macrophages and dendritic cells from human sources can be scarce or prone to donor variation we established an efficient method to generate induced pluripotent stem cell derived monocytes that in turn could be differentiated into both macrophages and dendritic cells. We used RNA sequencing to profile these from multiple differentiation runs (n=3) and multiple monocyte harvests (n=3-4) and compared them to their blood derived counterparts, blood derived monocyte, monocyte derived macrophages and moncyte derived dendritic cells (from 3 donors).
