Project description:Intact nuclei from an asynchronous population of W303 Saccharomyces cerevisiae in log-phase growth were subjected to a 16-minute DNase I digestion (0.1 U/μL) at 37 °C. DNA was then recovered, and single-end Illumina sequencing libraries were prepared using the Crawford DNase-seq method (Song and Crawford, 2010).
Project description:Proteomic analysis of the extracellular matrix of Saccharomyces cerevisiae W303-1A Wt and the isogenic mutant strain gup1Δ during the development of multicellular overlays.
Project description:Transcriptional profiling of Saccharomyces cerevisiae cells comparing the W303-1A wildtype with the W303-1A double mutant for MSN2 and MSN4 during zinc deficient conditions Keywords: Genetic modification with zinc limitation
Project description:Intact nuclei from an asynchronous population of W303 Saccharomyces cerevisiae in log-phase growth were subjected to a 16-minute DNase I digestion (0.1 U/μL) at 37 °C. DNA was then recovered, and single-end Illumina sequencing libraries were prepared using the Crawford DNase-seq method (Song and Crawford, 2010). Two biological replicates of DNase-seq were sequenced in single-end mode on an Illumina HiSeq 2000.
Project description:We performed single-cell RNA sequencing (scRNA-seq) of Saccharomyces cerevisiae cells across 20 environmental conditions using a lab w303 strain (ancestral) and an evolved strain grown in KCl under continuous directed evolution (derived). Cells were grown in each of 20 stress environments (18 for the derived strain) to log phase and harvested for scRNA-seq using the 10x Genomics Chromium platform. This dataset enables comparison of the variances in transcriptional responses across environments and between ancestral and evolved strains at single-cell resolution to reveal an adaptive hierarchy in stress response prioritization that collapses under continuous selection in a constant environment