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Arabidopsis thaliana

ABSTRACT: Differentially expressed small RNAs in Arabidopsis galls formed by Meloidogyne javanica

PROVIDER: PRJNA291520 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2135660.fastq.gz	Fastqsanger.gz
	SRR2135661.fastq.gz	Fastqsanger.gz
	SRR2135656.fastq.gz	Fastqsanger.gz
	SRR2135657.fastq.gz	Fastqsanger.gz
	SRR2135658.fastq.gz	Fastqsanger.gz

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Methylome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 14 days post infection

Project description:The goal of this study is to compare the methylation state of galls formed in Arabidopsis roots infected by Meloidogyne javanica at 14 days post infection compared to uninfected root segments (RC).

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Methylome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 3 days post infection

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Transcriptome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 3 days post infection

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Transcription profiling of Arabidopsis thaliana ematode induced early giant cells and normalvascular root cells.

Project description:Giant cells (GCs)formed by meloidogyne javanica 3 days post infection were isolated by microaspiration from the Arabidopsis Col0 galls. Three independent biological replicates were used for hybridisation. The controls for comparisons were vascular cylinder tissues from uninfected root segments at equivalent positions to the galls in the infected roots. RNA extracted directly from either the controls or the GCs were amplified two times with a RNA polimerase linear amplification based approach

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Characterization of small RNAs expressed in roots and galls from Arabidopsis thaliana induced by the plant parasitic nematodes Meloidogyne incognita

Project description:By sequencing small RNAs from uninfected Arabidopsis roots and from galls seven and 14 days post infection with Meloidogyne incognita, we sequenced by SOLiD technology the RNA fraction below 50nt. We identified 24 miRNAs differentially expressed in gall as putative regulators of gall development.

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Methylome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 3 days post infection

Project description:Methylome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 3 days post infection

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Transcriptome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 3 days post infection

Project description:Transcriptome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 3 days post infection

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Methylome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 14 days post infection

Project description:Methylome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 14 days post infection

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Down-regulation of gene expression is a conserved and distinctive feature required for early development of nematode-induced Giant Cells in tomato

Project description:Root-knot nematodes (RKN; Meloidogyne spp.) are sedentary parasites that affect a high variety of plants with a negative impact in the production of crops such as tomato. The infective RKN induces in the roots 4-7 highly specialized feeding cells (giant cells, GCs) developed into a hypertrophy cellular tissue or visible root swelling called “gall”. During GCs differentiation drastic alterations in genes expression occurs. However, information on genome-wide transcriptional profiles specifically in GCs is still lacking. With the aim to identify potential targets specifically regulated in GCs, we performed a temporal and spatial differential transcription profiling of tomato (Solanum lycopersicum), during the course of Meloidogyne javanica infection hybridizing TOM1 microarrays with hand-dissected galls at 1, 3, 7 and 14 days post-infection (dpi) and GCs exclusively isolated by LASER CAPTURE MICRODISSECTION (LCM) at 3 and 7 dpi. A GCs and galls comparison, at the same stage of development, reveals clear differences between their transcriptional patterns. For this purpose, a fast and efficient method to isolate LCM GCs from cryopreserved galls in the earliest differentiation state described to date: 48-72-hour post-infection (3 dpi), allowing good RNA preservation, was developed.

2012-06-15 | GSE30048 | GEO

Characterization of small RNAs expressed in roots and galls from Arabidopsis thaliana induced by the plant parasitic nematodes Meloidogyne incognita

Project description:Characterization of small RNAs expressed in roots and galls from Arabidopsis thaliana induced by the plant parasitic nematodes Meloidogyne incognita

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