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Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages

ABSTRACT: Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages

PROVIDER: PRJNA293705 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2180250_1.fastq.gz	Fastqsanger.gz
	SRR2180250_2.fastq.gz	Fastqsanger.gz
	SRR2180245_1.fastq.gz	Fastqsanger.gz
	SRR2180245_2.fastq.gz	Fastqsanger.gz
	SRR2180246_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages. [miRNA]

Project description:Hepatic ischemia reperfusion injury is a dynamic process consisting of two stages: ischemia and reperfusion, and triggers a cascade of physiological and biochemical events. Given the important role of microRNAs in regulating gene expression, we analyzed gene expression changes in mouse livers at sham control, ischemia stage, and reperfusion stage. We generated global expression profiles of microRNA and mRNA genes in mouse livers subjected to ischemia reperfusion injury at the three stages, respectively. Comparison analysis showed that reperfusion injury had a distinct expression profile whereas the ischemia sample and the sham control were clustered together. Consistently, there are 69 differentially expressed microRNAs between the reperfusion sample and the sham control whereas 28 differentially expressed microRNAs between the ischemia sample and the sham control. We further identified two modes of microRNA expression changes in ischemia reperfusion injury. Functional analysis of both the differentially expressed microRNAs in the two modes and their target mRNAs revealed that ischemia injury impaired mitochondria function, nutrient consumption, and metabolism process. In contrast, reperfusion injury led to severe tissue inflammation that is predominantly an innate-immune response in the ischemia reperfusion process. Our staged analysis of gene expression profiles provides new insights into regulatory mechanisms of microRNAs in mouse hepatic ischemia reperfusion injury.

2015-10-01 | GSE72314 | GEO

Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages. [mRNA]

2015-10-01 | GSE72312 | GEO

Proteomics analysis of different stages during mouse hepatic ischemia-reperfusion injury

Project description:6-plex TMT-based quantitative proteomics was used to analysis the proteomic profile in different stages of mouse hepatic ischemia-reperfusion injury.

2017-11-23 | PXD007827 | Pride

Transcriptional analysis on ischemia-reperfusion injury of mouse normal or steatotic livers

Project description:More and more steatotic livers undergo resection or transplantation but they exhibit higher susceptibility to ischemia-reperfusion injury (IRI), which results in increased perioperative complication morbidity and mortality. To investigate the underlying mechanisms, we conducted RNA-seq with liver samples derived from mice that were fed either high-fat-diet (HFD) or normal diet (ND) and experienced either IRI or sham operation.

2025-12-04 | GSE279693 | GEO

Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages.

Project description:Hepatic ischemia-reperfusion injury is a dynamic process consisting of two stages: ischemia and reperfusion, and triggers a cascade of physiological and biochemical events. Given the important role of microRNAs in regulating gene expression, we analyzed gene expression changes in mouse livers at sham control, ischemia stage, and reperfusion stage. We generated global expression profiles of microRNA and mRNA genes in mouse livers subjected to ischemia-reperfusion injury at the three stages, respectively. Comparison analysis showed that reperfusion injury had a distinct expression profile whereas the ischemia sample and the sham control were clustered together. Consistently, there are 69 differentially expressed microRNAs between the reperfusion sample and the sham control whereas 28 differentially expressed microRNAs between the ischemia sample and the sham control. We further identified two modes of microRNA expression changes in ischemia-reperfusion injury. Functional analysis of both the differentially expressed microRNAs in the two modes and their target mRNAs revealed that ischemia injury impaired mitochondrial function, nutrient consumption, and metabolism process. In contrast, reperfusion injury led to severe tissue inflammation that is predominantly an innate-immune response in the ischemia-reperfusion process. Our staged analysis of gene expression profiles provides new insights into regulatory mechanisms of microRNAs in mouse hepatic IR injury.

| S-EPMC4747576 | biostudies-literature

Transcriptional analysis on ischemia-reperfusion injury of mouse normal or steatotic livers

Project description:Transcriptional analysis on ischemia-reperfusion injury of mouse normal or steatotic livers

| PRJNA1178563 | ENA

Transcriptional analysis on ischemia-reperfusion injury of mouse normal or steatotic livers

Project description:Transcriptional analysis on ischemia-reperfusion injury of mouse normal or steatotic livers

| PRJNA1174092 | ENA

Pan-caspase inhibition during normothermic machine perfusion of discarded livers mitigates ischemia-reperfusion injury

Project description:Access to liver transplantation is limited by a significant organ shortage. The recent introduction of machine perfusion technology allows surgeons to monitor and assess ex situ liver function prior to transplantation. However, many donated organs are of inadequate quality for transplant, though opportunities exist to rehabilitate organ function with adjunct therapeutics during normothermic machine perfusion. In this preclinical study, we targeted the apoptosis pathway as a potential method of improving hepatocellular function. Treatment of discarded human livers during normothermic perfusion with an irreversible pan-caspase inhibitor, emricasan, resulted in significant mitigation of ischemia-reperfusion injury at both the transcriptional and protein level. This was evidenced by significantly decreased circulating levels of the pro-inflammatory cytokines, interleukin-6, interleukin-8, and interferon-gamma, compared to control livers. Untreated livers also demonstrated transcriptional changes notable for enrichment in pathways involved in innate immunity, leukocyte migration, and cytokine-mediated signaling. Targeting of unregulated apoptosis may represent a viable therapeutic intervention for rehabilitating liver hepatocellular function during machine perfusion.

2022-07-11 | GSE202565 | GEO

Ischemia reperfusion injury (IRI) following syngeneic lung transplantation

Project description:Ischemia reperfusion injury (IRI) continues to be an important factor contributing to long-term outcomes after transplantation. Here, utilizing a murine model of orthotopic left lung transplantation, we collected single cell RNA sequencing data from naive lungs and syngeneic lung grafts at various time points up to 30 days.

2026-01-13 | GSE276158 | GEO

The effect of age on interstitial cells following renal ischemia reperfusion injury

Project description:Single Cell RNA-SEQ of Murine Kidney post Ischemia Reperfusion Injury demonstrates heterogeneous mesencymal cells post injury and recapitulates novel markers with transcipts

2021-01-01 | GSE140010 | GEO

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