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A transcription factor pulse can prime chromatin for heritable transcriptional memory

ABSTRACT: A transcription factor pulse can prime chromatin for heritable transcriptional memory

PROVIDER: PRJNA294144 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2192250.fastq.gz	Fastqsanger.gz
	SRR2192260.fastq.gz	Fastqsanger.gz
	SRR2192280.fastq.gz	Fastqsanger.gz
	SRR2192290.fastq.gz	Fastqsanger.gz
	SRR2192251.fastq.gz	Fastqsanger.gz

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A transcription factor pulse can prime chromatin for heritable transcriptional memory [ChIPseq]

Project description:To investigate the epigenetic control of long-term transcriptionnal memory (LTTM) we used a trans-differentiation system where Bcell are reprogramed into macrophages upon induction of the transcription factor Cebpa. We performed ChIPseq in untreated Bcells, after 12h of Cebpa induction and after 6days of “wash” of Cebpa, to see if any proteins/histone marks are retained.

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A transcription factor pulse can prime chromatin for heritable transcriptional memory [RNAseq]

Project description:To indentify genes displaying long-term transcriptionnal memory (LTTM) we used a trans-differentiation system where Bcell are reprogramed into macrophages upon induction of the transcription factor Cebpa. We performed RNAseq in untreated Bcells, after 12h of Cebpa induction and after 6days of “wash” of Cebpa, to see if any genes respond more/faster in a second stimulation than in the first.

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A Transcription Factor Pulse Can Prime Chromatin for Heritable Transcriptional Memory.

Project description:Short-term and long-term transcriptional memory is the phenomenon whereby the kinetics or magnitude of gene induction is enhanced following a prior induction period. Short-term memory persists within one cell generation or in postmitotic cells, while long-term memory can survive multiple rounds of cell division. We have developed a tissue culture model to study the epigenetic basis for long-term transcriptional memory (LTTM) and subsequently used this model to better understand the epigenetic mechanisms that enable heritable memory of temporary stimuli. We find that a pulse of transcription factor CCAAT/enhancer-binding protein alpha (C/EBPα) induces LTTM on a subset of target genes that survives nine cell divisions. The chromatin landscape at genes that acquire LTTM is more repressed than at those genes that do not exhibit memory, akin to a latent state. We show through chromatin immunoprecipitation (ChIP) and chemical inhibitor studies that RNA polymerase II (Pol II) elongation is important for establishing memory in this model but that Pol II itself is not retained as part of the memory mechanism. More generally, our work reveals that a transcription factor involved in lineage specification can induce LTTM and that failure to rerepress chromatin is one epigenetic mechanism underlying transcriptional memory.

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A transcription factor pulse can prime chromatin for heritable transcriptional memory

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:A transcription factor pulse can prime chromatin for heritable transcriptional memory [RNAseq]

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Project description:A transcription factor pulse can prime chromatin for heritable transcriptional memory [ChIPseq]

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A histone hyperacetylation pulse induces cellular memory of gene expression and 3-dimensional genome folding [microC_3]

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