Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA294309 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Isoprene is a well-studied volatile hemiterpene that protects plants from abiotic stress through mechanisms that are not fully understood. The antioxidant and membrane stabilizing potential of isoprene are the two most commonly invoked mechanisms. However, isoprene also affects phenylpropanoid metabolism, suggesting an additional role as a signaling molecule. In this study, microarray based gene expression profiling reveals widespread transcriptional reprogramming of Arabidopsis thaliana plants fumigated for 24 hrs with a physiologically relevant concentration of isoprene. Functional enrichment analysis of fumigated plants revealed enhanced heat- and light-stress-responsive processes in response to isoprene. Isoprene induced a network enriched in ERF and WRKY transcription factors, which may play a role in stress tolerance. The isoprene-induced upregulation of phenylpropanoid biosynthetic genes was specifically confirmed using quantitative reverse transcription polymerase chain reaction. These results support a role for isoprene as a signaling molecule, in addition to its possible roles as an antioxidant and membrane thermoprotectant.
2015-09-01 | GSE72547 | GEO
Project description:Isoprene is a well-studied volatile hemiterpene that protects plants from abiotic stress through mechanisms that are not fully understood. The antioxidant and membrane stabilizing potential of isoprene are the two most commonly invoked mechanisms. However, isoprene also affects phenylpropanoid metabolism, suggesting an additional role as a signaling molecule. In this study, microarray based gene expression profiling reveals widespread transcriptional reprogramming of Arabidopsis thaliana plants fumigated for 24 hrs with a physiologically relevant concentration of isoprene. Functional enrichment analysis of fumigated plants revealed enhanced heat- and light-stress-responsive processes in response to isoprene. Isoprene induced a network enriched in ERF and WRKY transcription factors, which may play a role in stress tolerance. The isoprene-induced upregulation of phenylpropanoid biosynthetic genes was specifically confirmed using quantitative reverse transcription polymerase chain reaction. These results support a role for isoprene as a signaling molecule, in addition to its possible roles as an antioxidant and membrane thermoprotectant. Plants were held at 23 °C for 24 hours and then held at 40 °C for 24 hours, either in the presence or absence of 20 PPM isoprene during the entire 48 hours. Leaf samples were taken at the end of both 24 hour treatment periods. Each of the 4 resulting conditions was replicated 3 times.
2015-09-01 | E-GEOD-72547 | biostudies-arrayexpress
Project description:Genetically engineering Arabidopsis thaliana to express Isoprene Synthase (ISPS) leads to changes in expression of genes assoiated with many growth regulator signaling pathways and signaling networks involved in abiotic and biotic stress responses.
2019-05-15 | GSE121674 | GEO
Project description:Kinetic model of extended MEP pathway in Arabidopsis thaliana for isoprene.
This model was used for determining total optimization potential (TOP) of isoprene production in Arabidopsis thaliana using Spacescanner (https://github.com/atiselsts/spacescanner). This model was used to make designs D, E and F. An optimization task in COPASI can be perfomed using this model by accesing COPASI/Tasks/Optimization. Parameters which will be changed during the optimization and the optimization constraints can be selected.
More description in
Total optimization potential (TOP) approach based constrained design of isoprene and cis-abienol production in A. thaliana,
Authors: Katrina D. Neiburga, Reinis Muiznieks, Darta M. Zake, Agris Pentjuss, Vitalijs Komasilovs, Johann Rohwer, Alain Tissier, Egils Stalidzans
2023-02-04 | MODEL2206090003 | BioModels
Project description:Kinetic model of extended MEP pathway in wild-type Arabidopsis thaliana.
This model was used for the Parameter Estimation task in COPASI. Reactions for isoprene and cis-abienol synthesis are included but made inactive by setting their k=0. A new Parameter Estimation task can be performed by accesing COPASI/Tasks/Parameter Estimation. Notes about the parameters used in the model can be found in SupplModPar.
More description in
Total optimization potential (TOP) approach based constrained design of isoprene and cis-abienol production in A. thaliana,
Authors: Katrina D. Neiburga, Reinis Muiznieks, Darta M. Zake, Agris Pentjuss, Vitalijs Komasilovs, Johann Rohwer, Alain Tissier, Egils Stalidzans
2023-02-04 | MODEL2206090001 | BioModels
Project description:Kinetic model of extended MEP pathway in Arabidopsis thaliana for cis-abienol production.
This model was used for determining total optimization potential (TOP) of cis-abienol production in Arabidopsis thaliana using Spacescanner (https://github.com/atiselsts/spacescanner). This model was used to make designs A, B and C. An optimization task in COPASI can be perfomed using this model by accesing COPASI/Tasks/Optimization. Parameters which will be changed during the optimization and the optimization constraints can be selected.
More description in
Total optimization potential (TOP) approach based constrained design of isoprene and cis-abienol production in A. thaliana,
Authors: Katrina D. Neiburga, Reinis Muiznieks, Darta M. Zake, Agris Pentjuss, Vitalijs Komasilovs, Johann Rohwer, Alain Tissier, Egils Stalidzans
2023-02-04 | MODEL2206090002 | BioModels
Project description:This study aims to identify genes which help to understand similar underlying mechanism in the response to shade and wounding in Arabidopsis thaliana plants.
2022-05-12 | GSE133252 | GEO
Project description:Transcriptional profiling of Arabidopsis thaliana 12-days old seedlings comparing Col-0 wild type with transgenic plants with altered expression of dual-targetting plastid/mitochondrial organellar RNA-polymerase RPOTmp. Transgenic plants used for experiment were: overexpressor plants obtained by transformation of Col-0 WT plants with genetic constructs created in [Tarasenko et al., 2016] contained catalytic part of RPOTmp enzyme with transit peptides of RPOTm (mitochondrial) and RPOTp (plastid) by agrobacterial transformation; plants with complementation of RPOTmp functions in mitochondria or chloroplasts obtained from transformation of GABI_286E07 rpotmp knockout-mutant plants with genetic constructs created in [Tarasenko et al., 2016]. Goal was to determine the effects of RPOTmp knockout/overexpression on global Arabidopsis thaliana gene expression.
2023-12-25 | GSE251661 | GEO
Project description:Photoperiod is a circannual signal measured by biological systems to align growth and reproduction with the seasons. To understand the effect of photoperiod of gene expression in Arabidopsis thaliana in the absence of exogenous sugar under constant light intensity, we performed time course mRNA-seq analysis on 13-day old seedlings across three photoperiods with triplicates to identify photoperiod-regulated genes.
2023-08-15 | GSE221846 | GEO
Project description:Isoprene is a small lipophilic molecule synthetized at chloroplast level and released into the atmosphere. Isoprene-emitting plants are believed to be better protected against abiotic stresses (i.e. temperature, oxidative stress, drought). The mechanism of action of isoprene is still under debate. In this study we compared the physiological responses and proteomic profile of isoprene-emitting (ISPS) and non-emitting (WT and EV) Arabidopsis thaliana plants subjected to a moderate water stress and in control conditions. Our aim was to investigate if isoprene-emitting plants displayed a different proteomic profile which may help explain how isoprene protects plants from stresses. Only ISPS plants were able to maintain the same photosynthesis and fresh weight in water stress and in control condition. To better understand the molecular mechanism underlying isoprene emission, we performed a LC-MS/MS-based proteomic analysis to explore the changes in protein abundance between WT and EV both under control and moderate water stress conditions. Our data suggest that isoprene exerts its protective mechanism at different levels rather than on a single mode, triggering significant changes in chloroplast protein profiles, but also playing important roles in modulating signaling and hormone pathways and even membrane trafficking.
2022-05-20 | PXD025069 | Pride