Project description:Human peripheral blood mononuclear cells (PBMCs) from healthy donors were stimulated in vitro with a panel of commonly used immune stimuli and profiled using single‑cell RNA sequencing. The resulting dataset contains single-cell transcriptomic profiles of PBMCs across multiple donors and stimulation conditions. This resource enables comparative analysis of gene expression across immune cell populations and stimulation conditions and provides a reference for comparison with disease-associated immune signatures.
Project description:Primary human peripheral blood mononuclear cells were isolated from full blood by standard ficoll centrifugation. Cells were washed and processed immediately.
Project description:Human peripheral blood mononuclear cells (PBMCs) from healthy, de-identified donors were stimulated in vitro with a subset of immune stimuli and profiled by bulk RNA sequencing following fluorescence-activated cell sorting (FACS). Following a 48-hour assay, major immune cell populations were isolated, including monocytes, CD4+ T cells, CD8+ T cells, and B cells, and subjected to RNA-seq. This dataset captures cell type-specific transcriptional responses to B cell stimulator cells (BCS), CytoStim™, lipopolysaccharide (LPS), and IFN-α, alongside unstimulated baseline controls.
Project description:Transcriptome profiling was conducted on 2 replicate samples of total peripheral blood mononuclear cells (PBMC) and isolated PBMC subsets.
Project description:Ankylosing spondylitis (AS) is an inflammatory arthritis of the axial skeleton that predominantly affects young men. HLA-B27 has remained the major genetic risk factor in AS. Recently, more non-MHC genes has been discoverd to be involved in AS pathogenesis, especially IL-23 signalling pathway related genes. We performed a proteomic study of the peripheral blood mononuclear cells from AS patients and healthy donors.