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Daktulosphaira vitifoliae

ABSTRACT: Daktulosphaira vitifoliae overview

PROVIDER: PRJNA294982 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2051480_1.fastq.gz	Fastqsanger.gz
	SRR2051480_2.fastq.gz	Fastqsanger.gz
	SRR2294730_1.fastq.gz	Fastqsanger.gz
	SRR2294730_2.fastq.gz	Fastqsanger.gz
	SRR2294731_1.fastq.gz	Fastqsanger.gz

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Daktulosphaira vitifoliae

Project description:Daktulosphaira vitifoliae Raw sequence reads

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The salivary gland proteome of root-galling grape phylloxera (Daktulosphaira vitifoliae Fitch) feeding on Vitis spp.

Project description:Herbivory plant-parasite interactions depend on the delivery of effector molecules by the invading insect species. Sedentary gall forming insects, such as grape phylloxera (Daktulosphaira vitifoliae FITCH, Phylloxeridae) secrete multiple effectors into host plant tissues that alter host cellular functions to the benefit of the insect. Analyses revealed 420 putative ‘DvEffectors’ were detected in salivary glands, dissected from root-feeding vs. starving D. vitifoliae larvae reared on Teleki 5C (V. berlandieri x V. riparia) under controlled growth conditions (25±3°C, 60% rH) by proteomic mass spectrometry and in-silico secretory prediction. Sixty-two conserved DvEffectors were shared with the aphid species A. pisum, M. persicae and R. padi including candidate effector proteins involved in feeding site establishment, plant defence suppression and nutrient uptake

2020-07-22 | PXD014663 | Pride

Daktulosphaira vitifoliae

Project description:The 1KITE project: evolution of insects

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Daktulosphaira vitifoliae

Project description:Transcriptome and DNA methylome of Grape Phylloxera During Interaction with Different Host Grapevines

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Project description:Daktulosphaira vitifoliae RNA sequencing

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Project description:Daktulosphaira vitifoliae RNA sequencing

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Potential Global Distribution of Daktulosphaira vitifoliae under Climate Change Based on MaxEnt.

Project description:Grape phylloxera, Daktulosphaira vitifoliae, is a small, invasive, sap-sucking pest that is widely present in most viticulture regions all over the world. It is originally from North America and feeds on grapevine roots and leaves. In the current study, the potential distribution area of the leaf-feeding population was investigated with MaxEnt based on population occurrence data under different environmental variables. Results suggested that under current climatic conditions, Europe, East and North China, Japan, the Eastern USA, Uruguay, and the Southeast of South America are highly suitable areas for the occurrence of phylloxera leaf populations. The results showed that isothermality and precipitation of coldest quarter were major factors which contribute more than 60% of the model under current climate conditions. Our results provide important information for governmental decision makers and famers to develop control and management strategies against D. vitifoliae, and can also be used as a reference for studies on other invasive pest.

| S-EPMC8069807 | biostudies-literature

Daktulosphaira vitifoliae strain:Pcf7

Project description:Transcriptome of leaf- and root-feeding forms of grapevine phylloxera

| PRJNA294954 | ENA

A diagnostic LAMP assay for the destructive grapevine insect pest, phylloxera (Daktulosphaira vitifoliae).

Project description:Grape phylloxera (Daktulosphaira vitifoliae) is a destructive insect pest of grapevines that is highly invasive worldwide, despite strict biosecurity containment measures in place at farm and regional levels. Current phylloxera identification by visual inspection and laboratory-based molecular methods is time-consuming and costly. More rapid and cost-effective methods for identification of this pest would benefit industry, growers, and biosecurity services. Loop mediated isothermal amplification (LAMP) is a new portable technology available for rapid and accurate in-field molecular diagnostics. This study outlines the development of a new LAMP assay to enable the identification of phylloxera specimens. New LAMP primers were developed to specifically amplify phylloxera mitochondrial DNA (5'-COI), which we have shown is effective as a DNA barcode for identification of phylloxera, using LAMP technology. Positive LAMP reactions, containing phylloxera DNA, amplified in less than twelve minutes with an anneal derivative temperature of approximately 79 °C to 80 °C compared to a newly designed synthetic DNA (gBlock) fragment which had an anneal derivative temperature of 82 °C. No LAMP amplification was detected in any of the non-target species tested, i.e. no false-positive identification resulted for these species. We also successfully optimised a non-destructive DNA extraction procedure, HotSHOT "HS6", for use in the field on phylloxera adults, nymphs and eggs, to retain physical specimens. DNA extracted using this method was also suitable for species and genotype molecular identification methods, such as DNA barcoding, qPCR and microsatellite genotyping. The new LAMP assay provides a novel visual molecular tool for accurate diagnostics of phylloxera in the laboratory and field.

| S-EPMC7718921 | biostudies-literature

Project description:Grape phylloxera (Daktulosphaira vitifoliae Fitch) transcriptome between biotypes

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