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Schizosaccharomyces pombe

ABSTRACT: Regulation of mRNA Levels by Suicidal Introns that Induce Nuclear Decay

PROVIDER: PRJNA296069 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2435860_1.fastq.gz	Fastqsanger.gz
	SRR2435860_2.fastq.gz	Fastqsanger.gz
	SRR2435861_1.fastq.gz	Fastqsanger.gz
	SRR2435861_2.fastq.gz	Fastqsanger.gz
	SRR2435862_1.fastq.gz	Fastqsanger.gz

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Regulation of mRNA Levels by Suicidal Introns that Induce Nuclear Decay

Project description:In eukaryotic cells, inefficient splicing is surprisingly common and leads to degradation of transcripts with retained introns. How pre-mRNAs are committed to nuclear decay is unknown. Here we uncover a mechanism by which intronic transcripts are targeted for nuclear degradation in fission yeast. Surprisingly, sequence elements within “suicidal” introns co-transcriptionally recruit the exosome adaptor Mmi1 not only to degrade unspliced precursor, but also to downregulate levels of the resulting mRNA. Under conditions permissive for fast splicing, Mmi1 is no longer recruited and negative expression regulation is relieved. This mechanism negatively regulates levels of the RNA-helicase DDX5/Dbp2 to ensure cell survival in response to stress. We propose that suicidal introns are maintained because they facilitate regulation of gene expression. We identify multiple novel Mmi1 targets including mRNAs, non-coding RNAs, and sn/snoRNAs. We suggest a general role in RNA regulation for Mmi1 beyond degradation of meiotic transcripts.

2015-11-19 | GSE73144 | GEO

Regulation of mRNA Levels by Suicidal Introns that Induce Nuclear Decay

Project description:In eukaryotic cells, inefficient splicing is surprisingly common and leads to degradation of transcripts with retained introns. How pre-mRNAs are committed to nuclear decay is unknown. Here we uncover a mechanism by which intronic transcripts are targeted for nuclear degradation in fission yeast. Surprisingly, sequence elements within âsuicidalâ introns co-transcriptionally recruit the exosome adaptor Mmi1 not only to degrade unspliced precursor, but also to downregulate levels of the resulting mRNA. Under conditions permissive for fast splicing, Mmi1 is no longer recruited and negative expression regulation is relieved. This mechanism negatively regulates levels of the RNA-helicase DDX5/Dbp2 to ensure cell survival in response to stress. We propose that suicidal introns are maintained because they facilitate regulation of gene expression. We identify multiple novel Mmi1 targets including mRNAs, non-coding RNAs, and sn/snoRNAs. We suggest a general role in RNA regulation for Mmi1 beyond degradation of meiotic transcripts. Two biological replicates of CRAC experiments (Control and Mmi1-HTP). Six RNAseq datasets in total: three biological replicates of wt and delta Mmi1 strain.

2015-11-19 | E-GEOD-73144 | biostudies-arrayexpress

Schizosaccharomyces pombe

Project description:Time dynamics of quantitative protein and mRNA levels reveals extensive translational regulation after stress

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Schizosaccharomyces pombe

Project description:Negative regulation of Mis17-Mis6 centromere complex by mRNA decay pathway and EKC/KEOPS complex in Schizosaccharomyces pombe

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Schizosaccharomyces pombe

Project description:Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe

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Schizosaccharomyces pombe

Project description:Expression data of nuclear mRNA export mutant rae1-167 cells

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Schizosaccharomyces pombe Puf3 interactome

Project description:Puf3 is a RNA-binding protein, a member of the conserved Puf-protein family. Combining different functional genomics data, we have analyzed the role of Puf3 in post-transcriptional gene regulation in S. pombe. We present data on Puf3 interacting proteins and regulatory mRNA targets.

2022-03-23 | PXD006318 | Pride

Schizosaccharomyces pombe

Project description:The histone variant H2A.Z promotes splicing of weak introns (array)

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Schizosaccharomyces pombe

Project description:The histone variant H2A.Z promotes splicing of weak introns (ChIP-Seq)

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Schizosaccharomyces pombe splicing microarray

Project description:Analysis of splicing defects in Schizosaccharomyces pombe upon chemical genetic inhibition of splicing kinases dsk1, lkh1, and prp4, as well as alanine-mutation of phosphorylated residues in the splicing factors bpb1, prp2, rsd1, srp1, srp2, usp101, usp103, sum3, prp22, cdc5, and cwf22. This study shows the splicing kinase dsk1 modulates splicing efficiency of introns with non-consensus splice sites, likely through phosphorylation of bpb1. Modulation of splicing efficiency of transcripts through kinase signaling pathways may afford the necessary flexibility to tune the gene expression profile in response to environmental and developmental cues.

2015-07-13 | GSE62752 | GEO

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