Project description:Background: Diabetic wounds are a major complication of diabetes mellitus and heal poorly due to persistent inflammation, fibroblast dysfunction, oxidative stress, mitochondrial injury, and impaired angiogenesis. Scutellarein (SCU) is a natural flavonoid with demonstrated anti-inflammatory, antioxidant, and fibroblast-relevant bioactivities, but its effects on diabetic wound repair and the underlying molecular mechanisms remain poorly defined. Methods: A streptozotocin-induced diabetic mouse cutaneous wound model was used to evaluate the effects of topical SCU treatment on wound closure, epithelial repair, and collagen deposition. In vitro, an H₂O₂-induced oxidative injury model in fibroblasts was employed to assess SCU-mediated protection on migration, viability, redox balance, and mitochondrial structure and function. Transcriptomics (RNA-seq) was performed to identify SCU-responsive molecular pathways, and SCU-P2RX1 molecular docking combined with ATP rescue experiments was used to interrogate a P2RX1–calcium axis. Results: SCU accelerated diabetic wound closure and improved epithelial gap, collagen deposition, fibroblast-like cell proliferation, and angiogenesis in vivo. In vitro, SCU promoted fibroblast migration, suppressed H₂O₂-induced cell death, reduced intracellular ROS, restored mitochondrial morphology and membrane potential, and preserved ATP production. RNA-seq revealed that SCU downregulated P2rx1 and enriched calcium-transport, mitochondrial, and wound-healing pathways. Molecular docking predicted SCU binding to P2RX1 with a docking score of ΔG = −9.2 kcal/mol, and ATP addition reversed the SCU-mediated suppression of calcium overload, mitochondrial depolarization, and inflammatory gene expression.
Project description:Based on the genome sequencing and gene annotation, we analyzed the alginate lyases and other genes related to alginate metabolism of strain Vibrio sp. C42, with the highest alginate-degrading activity. Combined with proteome measurement, we proposed its alginate metabolic pathways.
Project description:This genome-scale metabolic model (GEM) of Corynebacterium tuberculostearicum strain DSM 44922 (Taxon ID 38304) was initially built with CarveMe version 1.5.1 based on the genome assembly with NCBI accession GCF_013408445.1 and then underwent a series of careful semi-automatic and manual curation. It is the first model curated using the Python tool MCC for mass and charge curation.
Project description:We sequenced and analyzed the genome of a highly inbred miniature Chinese pig strain, the Banna Minipig Inbred Line (BMI). we conducted whole genome screening using next generation sequencing (NGS) technology and performed SNP calling using Sus Scrofa genome assembly Sscrofa11.1.