Project description:Characterization of gene expression levels with RNA-seq was performed on self-renewing (SR) and senescent (SEN) human adult adipose derived mesenchymal stem cells (hADSCs) using the Roche 454 pyrosequencing platform.
Project description:To investigate the profiles of mRNAs, lncRNAs and circRNAs in browning of human adipose derived stem cells (hADSCs), we obtained hADSCs from omentum adipose tissues of 5 patients on who were performed abdominal operation and derived them into brown adipocytes. Then we employed microarray profiling the expression of lncRNAs, circRNAs and mRNA of hADSCs and their derived brown adipocytes.
Project description:Human adipose derived stem cells (hADSCs) are an attractive multipotent stem cell source with therapeutic applicability in diverse fields for the repair and regeneration of acute and chronically damaged tissues.In the last few years there has been increased interest in hADSCs for tissue engineering applications; however, the molecular mechanisms especially the microRNAs underlying the regulation of hADSCs proliferation and differentiation are not fully understood. To this end, we profiled the global microRNA expression in hADSCs, fibroblasts and endothelial cells by using Exqion mercury LNATM (Exqion Inc) To identify the microRNAs significantly expressed in human adipose derived stem cells, eight samples were analyzed using Exqion mercury LNATM (Exqion Inc) including six hADSCs samples and human endothelial and fibroblast cells as controls.
Project description:Human adipose derived stem cells (hADSCs) are an attractive multipotent stem cell source with therapeutic applicability in diverse fields for the repair and regeneration of acute and chronically damaged tissues. In the last few years there has been increased interest in hADSCs for tissue engineering applications; however, the molecular mechanisms underlying the regulation of hADSCs proliferation and differentiation are not fully understood. In an effort to reveal the functions essential for this process, we performed microarray (Agilent 4x44K Whole Human Genome Microarray Kit) to profile the global gene expression in the hADSCs by including human endothelial and fibroblast cells as controls. To identify the genes significantly expressed in human adipose derived stem cells, eight samples were analyzed using Agilent whole human genome microarray including six hADSCs samples and human endothelial and fibroblast cells as controls.
Project description:Human adipose derived stem cells (hADSCs) are an attractive multipotent stem cell source with therapeutic applicability in diverse fields for the repair and regeneration of acute and chronically damaged tissues.In the last few years there has been increased interest in hADSCs for tissue engineering applications; however, the molecular mechanisms especially the microRNAs underlying the regulation of hADSCs proliferation and differentiation are not fully understood. To this end, we profiled the global microRNA expression in hADSCs, fibroblasts and endothelial cells by using Exqion mercury LNATM (Exqion Inc)
Project description:In this study, we have addressed how cellular senescence influences the immunomodulatory potential of human mesenchymal stem cells (hMSCs). We induced cell senescence in a panel of bone marrow-derived hMSC samples by means of gamma-irradiation, and performed both gene expression and miRNA microarray analyses on the untreated and senescent samples. We also compared the gene expression profile of untreated and senescent hMSCs with those obtained from several hMSCs samples used in an ongoing allogeneic clinical study of Graft Versus Host Disease (GVHD), of which their therapeutic efficacy is known. We have identified several genes (PLEC, C8orf48, TRPC4, and ZNF14) differentially expressed in senescent hMSCs that are similarly regulated in hMSC samples that did not show a therapeutic effect in the GVHD study. These genes might be useful as markers to evaluate the therapeutic potential of hMSCs used in future clinical studies. We compared the gene and miRNA expression profiles of untreated (WT) control bone marrow-derived hMSCs with the same primary cell lines 10 days after gamma-irradiation (SEN) and with several hMSCs samples used in a clinical stydy of GVHD. The samples used in the clinical study were classified in two groups, depending on whether they elicited a therapeutic response (G1) or not (G2). A total of four independent samples (biological replicates) were used for WT and SEN conditions. For the samples used in the clinical study, a total of five samples were used for the G1 group, and three samples for the G2 group.
Project description:Human adipose derived stem cells (hADSCs) are an attractive multipotent stem cell source with therapeutic applicability in diverse fields for the repair and regeneration of acute and chronically damaged tissues. In the last few years there has been increased interest in hADSCs for tissue engineering applications; however, the molecular mechanisms underlying the regulation of hADSCs proliferation and differentiation are not fully understood. In an effort to reveal the functions essential for this process, we performed microarray (Agilent 4x44K Whole Human Genome Microarray Kit) to profile the global gene expression in the hADSCs by including human endothelial and fibroblast cells as controls.
Project description:To understand the differentiation of ovarian cancer stem cells (CSCs), We derived two phenotypes of CSCs and identified the gene expression profiling. The CSCs were derived from Cp70 ovarian cancer cells and cultured in suspension and examined every day for sphere formation. Spheres were then dissociated and passaged at least eight times in 2 months to generate spheres, which are henceforth referred to as SR cells. The surface of SR-I was smooth regardless of the size, whereas SR-II was morula. SR-I could differentiate into multiple-lineage cell types under specific induction conditions. SR-I spheroids could differentiate to SR-II spheroids through epithelial-mesenchymal transition.The self-renewal was slower for SR-I than for SR-II.
Project description:Recent studies suggest that transient premature senescence is essential for tissue remodeling. Myocardial infarction (MI) induces extensive myocardial remodeling through fibroblast-driven extracellular matrix (ECM) production. However, the characteristics and functions of the senescent cells (Sen.) following MI-induced cardiac remodeling remains elusive. In the present study, we observed a gradual increment number of Sen. within the ischemic region over time following MI, peaking at day 7 post-MI, with a subsequent decline in both wild-type mice and p16Ink4a-CreERT2-mT/mG reporter mice within 4 weeks. Using lineage tracing in the p16 reporter mice, we found most of the transient Sen. transitioned to non-senescent state. Then we analyzed our single-nucleus (sn)-multiome and fluorescence-based (SPiDER-β-gal/p16-EGFP) spatial transcriptomics data from the infarcted heart on day 7 post-MI to identify the cellular composition of transient Sen. We also conducted the deconvolution of the Sen. in the integrated dataset using different computational techniques. Additionally, we generated a reference (query dataset) based on SPiDER-βGal/p16-EGFP positivity and mapped it back to the snMultiome dataset. Through all approaches, we found fibroblasts and the subpopulation late myofibroblasts (MF) constituted a major proportion of Sen. In the snMultiome dataset, we explored the features of senescent late MF through differentially expressed genes/peaks and transcriptional binding motif analysis, and found the senescent late MF exhibited enhanced contractile properties and reduced ECM production capability compared with non-senescent late MF. These findings were supported by in vitro experiments showing that ischemia-induced senescent MF exhibited reduced soluble collagen production compared to TGF-β1-induced non-senescent MF. Additionally, in vivo studies revealed worsened cardiac function post-MI following senolytics administration compared to the vehicle group.
Project description:Recent studies suggest that transient premature senescence is essential for tissue remodeling. Myocardial infarction (MI) induces extensive myocardial remodeling through fibroblast-driven extracellular matrix (ECM) production. However, the characteristics and functions of the senescent cells (Sen.) following MI-induced cardiac remodeling remains elusive. In the present study, we observed a gradual increment number of Sen. within the ischemic region over time following MI, peaking at day 7 post-MI, with a subsequent decline in both wild-type mice and p16Ink4a-CreERT2-mT/mG reporter mice within 4 weeks. Using lineage tracing in the p16 reporter mice, we found most of the transient Sen. transitioned to non-senescent state. Then we analyzed our single-nucleus (sn)-multiome and fluorescence-based (SPiDER-β-gal/p16-EGFP) spatial transcriptomics data from the infarcted heart on day 7 post-MI to identify the cellular composition of transient Sen. We also conducted the deconvolution of the Sen. in the integrated dataset using different computational techniques. Additionally, we generated a reference (query dataset) based on SPiDER-βGal/p16-EGFP positivity and mapped it back to the snMultiome dataset. Through all approaches, we found fibroblasts and the subpopulation late myofibroblasts (MF) constituted a major proportion of Sen. In the snMultiome dataset, we explored the features of senescent late MF through differentially expressed genes/peaks and transcriptional binding motif analysis, and found the senescent late MF exhibited enhanced contractile properties and reduced ECM production capability compared with non-senescent late MF. These findings were supported by in vitro experiments showing that ischemia-induced senescent MF exhibited reduced soluble collagen production compared to TGF-β1-induced non-senescent MF. Additionally, in vivo studies revealed worsened cardiac function post-MI following senolytics administration compared to the vehicle group.