Project description:RNA-seq analysis of Pseudomonas sp OST1909 exposed to various preparations of naphthenic acids samples led to the identiifcation of many NA-induced genes.
Project description:The whole proteome analysis of the Pseudomonas sp. FIP_A4 strain in presence and absence of fipronil was conducted to evaluate the differentially expressed enzymes that can play role in fipronil degradation.
Project description:We performed MIcroarray experiments on a total of 12 immune organs, including spleen (SP), bone marrow (BM), lymph node (LN) and Peripheral blood mononuclear cell (PBMC) and three tumor tissues including MC38, EL4 and LLC. 12 mice from four immune organs, including the spleen (SP), bone marrow (BM), lymph nodes (LN), and Peripheral blood mononuclear cell (PBMC), were isolated using routine experimental methods.9 mice were randomly divided into 3 groups and implanted subcutaneously(s.c.) with EL4 (2.0*10^5 /mice), MC-38 (1.0*10^6 /mice), and LLC-JSP-t2 (2.0*10^5 /mice) cells, respectively. All mice were sacrificed and tumor tissues were collected when the tumor volume reached 100 mm 3 . The tissues were ground up with liquid nitrogen and the lysates were stocked using Trizol for subsequent array analysis. RNA isolation and hybridization were performed by Capitalbio Technology Corporation according to the standard Affymetrix protocol .
