Project description:Here, we employed high-throughput sequencing to identify microRNAs in CMS and its maintainer fertile (MF) lines of Brassica juncea. We identified 197 known and 78 new candidate microRNAs during reproductive development of B. juncea. A total of 47 differentially expressed microRNAs between CMS and its maintainer fertile lines were discovered, according to their sequencing read number.
Project description:The BnCRY2aOE transgenics over-expressing a blue light photoreceptor (BnCRY2a) flowered earlier than wild-type plants by more than two weeks. To identify the downstream candidate genes involved in regulating the early flowering phenotype in the transgenics, a genome-wide microarray analysis of the transgenic plant vs. wild type was performed. The microarray data analysis revealed the differential up-regulation of many genes involved in flower development, cell growth and differentiation and hormone biosynthesis/signalling in comparison to wild-type.
