Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Brassica juncea seeds treated with thiourea under salinity stress

ABSTRACT: Effect of TU on the change in the expression profile under the salinity stress imposed to the seeds of Brassica juncea

ORGANISM(S): Brassica juncea

SUBMITTER: Ashish Srivastava

PROVIDER: E-MEXP-1903 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Shared Molecules

Only show the datasets with similarity scores above: 0.5

Threshold

0.5

Similar Datasets

Transcription profiling of mouse 6780 cell line derived from murine T-cell lymphomaTGFbeta dependent gene expression in T-cell lymphomas

Project description:The cell line "6780" derived from murine T-cell lymphoma was grown in RPMI medium 1640 with 10% FBS/1% penicillin/Streptomycin/1% l-glutamine/50 Î¼M 2-mercaptoethanol. Vector constructs containing a dominant negative mutant of the human TGF-beta receptor type II (pMSCV-TGFBR-IRES-GFP) or an empty vector (pMSCV-IRES-GF) were kindly provided by Martin Eilers Group, IMT, Marburg, Germany. Cells were incubated with retroviruses containing supernatants for 12 h at 32°C in media containing 4 ?g/ml polybrene. Cells then were expanded at 37°C for an additional 48 h, and GFP-expressing cells were purified by flow cytometry on a FACS Vantage (Becton Dickinson). All animal experiments were performed by following the guidelines from Administrative Panel on Laboratory Animal Care at Stanford University (protocol 8144). For transplantation experiment cells were washed once with PBS and 10x106 cells/site were injected subcutaneously (s.c.) into the flank of FVB/N syngeneic mice. Tumor growth was measured by using a caliper. To inactivate MYC expression and to assess for tumor regression, the drinking water was supplemented with 200 Î¼g/ml doxycycline. Tumors were harvested untreated or after 1, 3 and 4 days upon doxycycline treatment, respectively. Total RNA was isolated from snap-frozen tumor tissue using the RNeasy Kit including DNase-I digest (Qiagen, Valencia, USA) following the manufacturer's protocol.

2010-01-01 | E-MEXP-2233 | biostudies-arrayexpress

Transcription profiling of zebrafish embryos injected with a morpholino targeting LIN9

Project description:TÜAB zebrafish were maintained at 28.5 °C in in 1x Danieau solution (58 mM NaCl, 0.7 mM KCl, 0.4 mM MgSO4, 0.6 mM Ca (NO3)2, 5 mM HEPES, pH 7.6, 0.0001 % Methylene blue). Morpholinos (Gene Tools, Philomath, OR) were designed against Danio rerio lin-9 homolog (Genebank accession NM_001044946). The morpholino (MO) sequences were MO-E1 5´-GTTAGTTTTATTACTCACTCTCGTC-3´ and 5-base mismatch morpholino MO-E1mis 5´-GTTACTTTTAATACTGACTGTCCTC-3´. 7 ng of morpholinos were injected into one cell-stage embryos. 20 embryos per condition (MO E1; MO E1mis) were pooled for RNA purification 24 h post fertilization. Using the two color Quick-Amp Labeling Kit (Agilent; 5190-0444) 100 ng of total RNA were used for cDNA synthesis, mRNA amplification and labeling according to manufacturers instructions. Transcriptional profiling was done on a zebrafish oligo array (Agilent; G2519F AMADID 019161) in a 4 x 44K slide format and analyzed as described before.

2009-03-28 | E-MEXP-2100 | biostudies-arrayexpress

Transcription profiling of human U2OS cell line transfected with IZ-1 and 14 ARF expressing plasmids

Project description:U2OS cells were stably transfected with an ecotropic receptor expression plasmid. These cells were infected with retroviruses expressing MIZ-1 or MYC and subsequently superinfected with retroviruses expressing p14ARF. Selection was carried out 48h following superinfection and selected cells were harvested within 1 - 2 passages. For each condition around 2.5x10<superscript6> cells were pooled. Using the two color Quick-Amp labelling kit (Agilent, 5190-0444) 100ng of total RNA were used for cDNA synthesis, aRNA amplification and labelling according to manufacturer's instructions. Transcriptional profiling was done on a whole human genome oligo microarray (Agilent, G4112F, 014850) in a 4x44k slide format.

2009-11-01 | E-MEXP-2310 | biostudies-arrayexpress

Deregulated Myc expression induces addiction to AMPK-related kinase 5

Project description:U2OS cells were infected with shRNA vector targeting Ark5 or control vector, selected on puromycin for 48hrs, seeded on 5cm dishes at 20% confluency and harvested in triplicate 48hrs later

2012-03-01 | E-MEXP-3543 | biostudies-arrayexpress

Transcription profiling of mouse liver from a wild type strain fed on a diet supplemented with cholic acid for 7 days

Project description:Genome-wide changes in hepatic gene expression in wild-type mice on a diet supplemented with cholic acid, a naturally occurring bile acid

2009-05-08 | E-MEXP-2109 | biostudies-arrayexpress

Transcription profiling of mouse liver from wild type and Foxa2 knock out strains fed on standard diet

Project description:Transcriptional profiling of changes in global hepatic gene expression in mice lacking Foxa2 in the liver (Foxa2loxP/loxP;Alfp.Cre) on standard rodent diet

2009-05-01 | E-MEXP-2106 | biostudies-arrayexpress

Transcription profiling of mouse liver from wild type and Foxa2 knock out strains fed on a diet supplemented with cholic acid

Project description:Transcriptional profiling of changes in global hepatic gene expression in mice lacking Foxa2 in the liver (Foxa2loxP/loxP;Alfp.Cre) on a diet supplemented with cholic acid, a naturally occurring bile acid

2009-05-08 | E-MEXP-2107 | biostudies-arrayexpress

Transcription profiling of mouse embyronic fibroblasts from wild type and Lin9 knockout strains treated with 4-hydroxytestosterone

Project description:We used microarrays to analyze the role of Lin9 in gene expression. Experimental overall design: We prepared MEFs from embyos containing a conditional allele of Lin9 and a CreERT2 transgene. Transcriptional profiles of untreated and 4-OHT-treated MEFs were compared.

2010-03-31 | E-MEXP-2097 | biostudies-arrayexpress

Transcription profiling of Arabidopsis roots from miniyo mutant and wild type (Landsberg) plants

Project description:comparison of the transcription profile of roots of the Arabidopsis miniyo mutant with the corresponding wild type (accession Landsberg)

2009-12-01 | E-MEXP-2046 | biostudies-arrayexpress

Miz1 is required to maintain physiological levels of autophagic flux_1

Project description:Mice: knock-out of the Miz1 POZ-domain in brain (Miz1DPOZNes) and control mice. The RNA expression in cerebella of of old Miz1 delta POZ mice (1.5 years), old control mice (1.5 years), young Miz1 delta POZ mice (5.5 weeks) and young control mice (5.5 weeks) was compared

2013-10-01 | E-MEXP-3878 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data