Project description:Wastewater treatment plants (WWTPs) and Drinking water treatment plants (DWTPs) are critical points for public health for persistently remaining microorganisms after treatment may pose a risk. This study aimed to conduct microbial metagenomic analyses on waters from both DWTPs and WWTPs under the Istanbul Water and Sewerage Administration (ISKI). In this study a total of 52 samples were included, comprising 18 samples from DWTPs and 34 from WWTPs. All water samples underwent pre-isolation filtration. DNA isolation was conducted using filter material, followed by library preparation and sequencing on a NovaSeq 6000 instrument following the manufacturer's guidelines.
Project description:This study investigated microbial communities from two wastewater treatment plants (WWTPs) for their potential to degrade PET and investigated enzymes likely involved in its degradation. Activated sludge from urban and industrial WWTPs was analyzed using whole metagenome sequencing and metaproteomics to characterize biofilms and their expressed enzymes.