Project description:We used microarrays to investigate the whole genome gene expression level changes of LncRNAs in human Glioblastoma multiforme (GBM) and normal brain tissues, and try to find out some LncRNA associated with the tumorigenesis of GBM.
Project description:Glioblastoma (GBM) is the most common and lethal primary malignancy of the central nervous system in adult. In order to improve the diagnosis, prevention and treatment of GBM, the details of molecular mechanisms underlying the tumorigenesis and development needs to be clarified. This is a nalysis of glioblastoma tissues and matched adjacent normal brain tissues from 3 patients. Results provide insight into molecular mechanisms underlying the non-coding and coding genes interactions in glioblastoma. 3 human GBM tissues and the matched adjacent normal brain tissues were analyzed using microarray. The aberrant lncRNAs, miRNAs and mRNAs between the 2 groups were detected.
Project description:We used microarrays to investigate the whole genome gene expression level changes of LncRNAs in human Glioblastoma multiforme (GBM) and normal brain tissues, and try to find out some LncRNA associated with the tumorigenesis of GBM. The human LncRNA microarray analysis of 9 samples (5 GBM and 4 normal brain tissues) were completed. Total RNA from each sample was quantified and RNA integrity was assessed using standard denaturing agarose gel electrophoresis. Total RNA of each sample was used for labeling and array hybridization. Array scanning using the Agilent Scanner G250C. Scanned images were then imported into NimbleScan software (version 2.5) for expression data analysis. Differentially expressed LncRNAs were filtered out for further study.
