Haloferax volcanii and Haloferax mediterranei hybrid
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA327107 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR5198904.fastq.gz | Fastqsanger.gz | |||
| SRR5198905.fastq.gz | Fastqsanger.gz | |||
| SRR5198906.fastq.gz | Fastqsanger.gz | |||
| SRR5198907.fastq.gz | Fastqsanger.gz |
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Project description:Polyploidy, the phenomenon of having more than one copy of the genome in an organism, is common among haloarchaea. While providing short-term benefits for DNA repair, polyploidy is generally regarded as an "evolutionary trap" that by the notion of the Muller's ratchet will inevitably conclude in the species' decline or even extinction due to a gradual reduction in fitness. In most reported cases of polyploidy in archaea, the genetic state of the organism is considered as homoploidy i.e. all copies of the genome are identical. Here we demonstrate that while this is indeed the prevalent genetic status in the halophilic archaeon Haloferax volcanii, its close relative H. mediterranei maintains a prolonged heteroploidy state in a nonselective environment once a second allele is introduced. Moreover, a strong genetic linkage was observed between two distant loci in H. mediterranei indicating a low rate of homologous recombination while almost no such linkage was shown in H. volcanii indicating a high rate of recombination in the latter species. We suggest that H. volcanii escapes Muller's ratchet by means of an effective chromosome-equalizing gene-conversion mechanism facilitated by highly active homologous recombination, whereas H. mediterranei must elude the ratchet via a different, yet to be elucidated mechanism.
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Project description:Archaea are ubiquitous prokaryotes with a wide range of habitats, important roles in ecology, biotechnology and potentially even human health. Despite that, our understanding of archaeal cell biology is still rather limited, partially because the application of systems biology approaches is lacking behind the other domains of life. Here we introduce/announce the Archaeal Proteome Project (ArcPP), a community effort that aims for the comprehensive analysis of archaeal proteomes. Starting with the model archaeon Haloferax volcanii, we have re-analyzed more than 2 TB of MS result files (>20 Mio. spectra) using state-of-the-art bioinformatic tools, increasing peptide spectrum matches and leading to the secure identification of >3000 proteins. This dataset is part of the Archaeal Proteome Project dataset
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