Project description:To investigate the role of insulin-receptor substrate-1 (Irs-1) in the regulation of bone metabolism, we generated Irs-1-deficient mice (Irs1smla/smla). Using the highly sensitive miRNA array, we screened the differentially expressed miRNAs of 2-month-old Irs-1smla/smla mice, Irs-1+/smla mice and Irs-1+/+ mice. Three prediction algorithms (TargetScanS, miRanda, and PicTar) were used to identify the target genes of differentially expressed miRNAs. Furthermore, the quantitative real-time polymerase chain reaction, immunohistochemistry and dual luciferase reporter assay results showed that miR-342-3p is a specific regulator of collagen type I alpha 2 (Col1a2), which will give new insights in disclosing the mechanism of diabetic osteopathy.
Project description:To investigate the differences in microRNA expression profiles between fibrotic and normal livers, we performed microRNA microarrays for total RNA extracts isolated from mouse livers treated with carbontetrachloride (CCl4) or corn-oil for 10 weeks (n=3/group). MicroRNAs were considered to have significant differences in expression level when the expression difference showed more than two-fold change between the experimental and control groups at p<0.05. We found that 12 miRNAs were differentially expressed in CCl4-induced fibrotic liver. To induce chronic liver fibrosis, seven-week-old mice received 0.6 ml/kg body weight of carbon-tetrachloride (CCl4) dissolved in corn-oil by intraperitoneal (i.p.) injection, twice a week for 10 weeks (n=3). As a control, same number of mice was injected with equal volume of corn-oil for 10 weeks.
Project description:To describe the protein profile in hippocampus, colon and ileum tissue’ changing after the old faeces transplants, we adopted a quantitative label free proteomics approach.
Project description:Identification of differentially expressed genes in young (3 month old) versus aged (24 month old) mouse hematopoietic stem cells. Comparison of genes differentially expressed in hematopoietic stem cells of young mice with conditional deletion of mTOR within vascular endothelium.
Project description:Identification of differentially expressed genes in young (3 month old) versus aged (24 month old) mouse bone marrow derived endothelial cells. Comparison of genes differentially expressed in bone marrow derived endothelial cells of young mice with conditional deletion of mTOR within vascular endothelium.
