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ABSTRACT: Pisum sativum transcript sequencing

PROVIDER: PRJNA328997 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR3898730.fastq.gz	Fastqsanger.gz
	SRR3898740.fastq.gz	Fastqsanger.gz
	SRR3898731.fastq.gz	Fastqsanger.gz
	SRR3898732.fastq.gz	Fastqsanger.gz
	SRR3898723.fastq.gz	Fastqsanger.gz

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Project description:Seeds of Pisum sativum L. cv Alaska Early (purchased from the ‘Abundant Life Seed Foundation’, Port Townsend, USA) were equilibrated for approximately 5 weeks in tightly sealed boxes over 300 g-1 non-saturated LiCl (Hay et al. 2008), producing 60% relative humidity, in a temperature-controlled room (20 ± 1 ºC) until their water potential was stable at 12 % MC. Relative humidity was recorded with a Rotronic AWVC-D10 Hygropalm. These equilibrated seeds are referred to as 0 day ‘non-aged controls’. Following equilibration, accelerated ageing was carried out by placing seeds in sealed bottles at 50 °C until viability loss, thereafter referred to as ‘ageing’. Samples were taken at intervals after 8, 12, and 15 days of ageing. At each interval, germination tests were performed on 1 % water agar at 25 °C under warm white fluorescent light (15 micromol m-2 s-1) at a day / night cycle (8/16h). Germination was defined as radical emergence by at least 2 mm. To summarize, we have performed a transcriptional profiling of Pisum sativum seeds comparing 0 day ‘non-aged controls’ with 8, 12 and 15 days of artificially aged seeds.

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