Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Three dimensional culturing of HBMEC

PROVIDER: PRJNA344703 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR4299220.fastq.gz	Fastqsanger.gz
	SRR4299230.fastq.gz	Fastqsanger.gz
	SRR4299240.fastq.gz	Fastqsanger.gz
	SRR4299211.fastq.gz	Fastqsanger.gz
	SRR4299221.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 29

Similar Datasets

Three-dimensional Co-culturing Reveals Human Stem Cell-Derived Somatostatin Interneurons with Subclass Expression

Project description:Three-dimensional Co-culturing Reveals Human Stem Cell-Derived Somatostatin Interneurons with Subclass Expression

| PRJNA1304466 | ENA

Project description:three-phase three-dimensional electrode

| PRJNA661628 | ENA

Spheroid growth in ovarian cancer promotes gene expression involved in epithelial-mesenchymal transition, stem cell promotion, and multiple stress responses

Project description:In this study, we examine gene expression changes through microarray analysis in spheroid versus monolayer ovarian cancer cells treated with TGFβ to induce EMT. Interestingly, EMT, stress response, and stem cell differentiation pathways were all significantly affected by 3D growth. These findings support the hypothesis that three dimensional ovarian cell culturing is physiologically different from its monolayer counterpart.

2016-04-18 | GSE80373 | GEO

Synergistic effects between rutin and ascorbic acid in the protection of the proteome of 3D-cultured human skin fibroblasts from UVA radiation

Project description:This study demonstrates the cooperation of rutin and ascorbic acid in cytoprotective action against UVA induced changes in the context of proteins involved in gene expression, catalytic processes, antioxidant pathways, as well as signalling molecules including transmembrane channels. The main advantages of the work is application in research a three-dimensional (3D) cell culturing system providing the most epidermal-like conditions.

2020-11-20 | PXD018730 | Pride

Marinobufagenin regulates gene expression in human brain microvascular endothelial cells in vitro.

Project description:To understand the mechanism by which MBG increases permeability of human brain microvascular endothelial cell (HBMEC) monolayers, the primary cultures were treated with 10nM MBG for 12 h. RNA was isolated and analysed for gene expression on Agilent one color microarrays. Triplicate wells of HBMEC were treated with 10 nM MBG in DMSO or with just the drug vehicle for 12 h. RNA was isolated from each well and used to reverse transcribe and generate Cy3-labelled cRNA. Microarrays were hybridized, washed and scanned. Data were normalized and assessed for differential gene expression between MBG treated and control HBMEC.

2013-11-01 | E-GEOD-51351 | biostudies-arrayexpress

Streptococcus suis 05ZY: control vs contact with hBMEC 3 h

Project description:The interactions of Streptocococcus suis (S.suis) with human microvascular endothelial cells (hBMEC) are important process for S.suis passage across human blood brain barrier (BBB). S.suis has evolved precise mechanisms to alter gene expression depending on the distinct challenges posed by particular disease sites. Herein, a whole-genome DNA microarray was used to investigate the change of gene expression profile of S.suis after contact with hBMEC 3 h. comparison of RNA isolated from hBMEC-associated S.suis with RNA derived from control bacteria revealed significant differential changes for 175 S.suis genes including 123 up-regulated genes and 52 down-regulated genes at 3 h post infection. A cDNA microarray imprinted with 2156 genes representing about 98% of Streptococcus suis serotype 2 genome was used for transcriptome analysis . For two-sample (reference vs. test) microarray hybridization, Two-condition experiment, control vs contact with hBMEC 3 h, four replicates (two biological replicates, two technical replicates) at each condition.

2015-12-31 | E-GEOD-46962 | biostudies-arrayexpress

Streptococcus suis 05ZY: control vs contact with hBMEC 1 h

Project description:The interactions of Streptocococcus suis (S.suis) with human microvascular endothelial cells (hBMEC) are important process for S.suis passage across human blood brain barrier (BBB). S.suis has evolved precise mechanisms to alter gene expression depending on the distinct challenges posed by particular disease sites. Herein, a whole-genome DNA microarray was used to investigate the change of gene expression profile of S.suis after contact with hBMEC 1 h. comparison of RNA isolated from hBMEC-associated S.suis with RNA derived from control bacteria revealed significant differential changes for 219 S.suis genes including 131 up-regulated genes and 88 down-regulated genes at 1 h post infection. A cDNA microarray imprinted with 2156 genes representing about 98% of Streptococcus suis serotype 2 genome was used for transcriptome analysis . For two-sample (reference vs. test) microarray hybridization, Two-condition experiment, control vs contact with hBMEC 1 h, four replicates (two biological replicates, two technical replicates) at each condition.

2015-12-31 | E-GEOD-46961 | biostudies-arrayexpress

Streptococcus suis 05ZY: control vs contact with hBMEC 3 h

2015-12-31 | GSE46962 | GEO

Streptococcus suis 05ZY: control vs contact with hBMEC 1 h

2015-12-31 | GSE46961 | GEO

Project description:Three-dimensional geome study of procine livers

| PRJNA721459 | ENA