Project description:To determine HIF1a target genes in ATII cells, we transduced freshly isolated rat ATII cells with an adenoviral vector expressing either GFP or a constitutively active mutant HIF1a construct. After 24 hours, RNA was extracted and subjected to microarray.
Project description:To examine the role of estrogen in muscle, differentiated myoblastic C2C12 cells were transduced with adenoviruses expressing constitutively active estrogen receptor α mutant (Ad-caERα) or DsRed (Ad-DsRed) as a control. Microarray analysis showed that the mRNA level of nuclear receptor subfamily 4 group A member 1 (NR4A1) was upregulated by the Ad-caERα compared with the control Ad-DsRed.
Project description:Comparison of Gene expression profiling of granulosa cells treated with follicle stimulating hormone or constitutively active protein kinase A
Project description:Beta-cell proliferation is enhanced in the mice treated with with adenovirus containing constitutively active mutant of MEK1 (CAM mice) than mice treated control adenovirus. This microarray anaslysis was performed for the purpose of exploring the molecular mechanism thereby beta-cell proliferation is enhanced in L-MEK mice.
Project description:The Norway rat has important impacts on our life. They are amongst the most used research subjects, resulting in ground-breaking advances. At the same time, wild rats live in close association with us, leading to various adverse interactions. In face of this relevance, it is surprising how little is known about their natural behaviour. While recent laboratory studies revealed their complex social skills, little is known about their social behaviour in the wild. An integration of these different scientific approaches is crucial to understand their social life, which will enable us to design more valid research paradigms, develop more effective management strategies, and to provide better welfare standards. Hence, I first summarise the literature on their natural social behaviour. Second, I provide an overview of recent developments concerning their social cognition. Third, I illustrate why an integration of these areas would be beneficial to optimise our interactions with them.
Project description:BackgroundMurine kobuviruses (MuKV) are newly recognized picornaviruses first detected in murine rodents in the USA in 2011. Little information on MuKV epidemiology in murine rodents is available. Therefore, we conducted a survey of the prevalence and genomic characteristics of rat kobuvirus in Guangdong, China.ResultsFecal samples from 223 rats (Rattus norvegicus) were collected from Guangdong and kobuviruses were detected in 12.6% (28) of samples. Phylogenetic analysis based on partial 3D and complete VP1 sequence regions showed that rat kobuvirus obtained in this study were genetically closely related to those of rat/mouse kobuvirus reported in other geographical areas. Two near full-length rat kobuvirus genomes (MM33, GZ85) were acquired and phylogenetic analysis of these revealed that they shared very high nucleotide/amino acids identity with one another (95.4%/99.4%) and a sewage-derived sequence (86.9%/93.5% and 87.5%/93.7%, respectively). Comparison with original Aichivirus A strains, such human kobuvirus, revealed amino acid identity values of approximately 80%.ConclusionOur findings indicate that rat kobuvirus have distinctive genetic characteristics from other Aichivirus A viruses. Additionally, rat kobuvirus may spread via sewage.
Project description:RNA-Seq analysis was performed from human umbilical vein endothelial cells exposed to constitutively active HIF1a and HIF2a overexpression
