Project description:Pancreatic cancer is one of the most aggressive human malignancies with 7.1% five-year survival rate due to late-stage diagnosis and lack of effective treatment. A novel active compound to be useful for treating pancreatic cancer is absolutely demanded. Agaricus blazei (A. blazei) Murrill has been shown to have an anti-cancer activity, however, its specific efficacy and underlying mechanism is poorly understood. Here, we demonstrated that A. blazei hot water extract (AbE) showed significant growth inhibition of cultured pancreatic cancer cells with less suppressive effect on the normal human pancreatic duct epithelial cells. The pancreatic cancer cells committed G0/G1 cell cycle arrest and caspase-dependent apoptosis. Moreover, we uncovered significant alterations of global gene expression in the pancreatic cancer cells by AbE treatment. Signature of alteration of gene expression indicated that AbE induced repression of genes associated with kinetochore function, spindle formation, centromere maintenance, and cyclins and cyclin dependent kinases, which were essential for cell cycle progression. The gene expression analysis also showed upregulation of proapoptotic genes. These results indicate that AbE could be useful for treatment of pancreatic cancer and a good candidate to isolate potent active compound for development of novel drug for pancreatic cancer.
Project description:Comparing the expression profiles of the genes in response to 17b-estradiol (E2) and Agaricus blazei extract (ABE) Two-condition experiment, E2- or ABE-treated vs. control cells. 2 to 3 biological replicates, independently grown and harvested. Two to three replicates per array.
Project description:The effect on gene expression of an extract from a medicinal mushroom (Agaricus blazei Murill)were compared with the effect of lipopolysaccharides (LPS). Both are potent immunomodulators. The study used a monocyte cell line.
Project description:The edible mushroom Agaricus blazei Murill has immunomodulating and antiproliferative effects. In a clinical study 33 patients with multiple myeloma were randomized to receive treatment with Agaricus (16 patients) or placebo (17 patients) in addition to chemotherapy.
Project description:To investigate gene expression at different development stages of Agaricus blazei, we utilized Illumina Hiseq PE150 sequencing technology to obtain the transcriptome of mycelium and fruit body primodium
Project description:The edible mushroom Agaricus blazei Murill has immunomodulating and antiproliferative effects. In a clinical study 33 patients with multiple myeloma were randomized to receive treatment with Agaricus (16 patients) or placebo (17 patients) in addition to chemotherapy. Gene expression profiles were analyzed before and after treatment in 8 patients in the agaricus group and in 6 patients in the control group and the differences were calculated
