Project description:In this study we investigated the global proteomic response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2. For this porpouse HUVECs were incubated with SARS-CoV-2 at a multiplicity of infection (MOI) of 1 and after different hours post-infection (hpi) samples were submitted to the 50% tissue-culture infective dose assay (TCID50), proteomic analysis and RT-qPCR measurements.To perform the relative quantitative label-free proteomic analysis, cells exposed to SARS-CoV-2 or MOCK were submitted to protein extraction, trypsin digestion and the tryptic peptides were analyzed in a Q-Exactive mass spectrometer. Data analysis of the differently abundant proteins of each cell model revealed distinct cell responses.
Project description:Untargeted proteomics dataset of human dermal blood endothelial cells (HDBECs), human umbilical vein endothelial cells (HUVECs), human dermal lymphatic endothelial cells (HDLECs) and intestinal lymphatic endothelial cells (iLECs) in proliferation and quiescence.
Project description:Intra- and extracellular metabolomics dataset of human dermal blood endothelial cells (HDBECs), human umbilical vein endothelial cells (HUVECs), human dermal lymphatic endothelial cells (HDLECs) and intestinal lymphatic endothelial cells (iLECs) in proliferation and quiescence.
Project description:To understand the the effect of poly(lactic-co-glycolic acid) nanoparticles (NPs) encapsulating a fluorine contrast agent on human umbilical vein endothelial cells (HUVECs), we have employed whole genome microarray expression profiling. The NPs used in this study were prepared in our lab. HUVECs were ordered from Lonza (Cat# C2517A). HUVECs were incubated with NPs for 4 hours. After 4 h, the cells were washed couple of times to remove the NPs and gene expression profiles were detected after 20 h (total time: 24 h) and 7 d in the absence of NPs. HUVECs after 24 h without nanoparticles were used as controls.
Project description:To investigate machanism of miR-210-3p regulating angiogenic ability of human umbilical vein endothelial cells (HUVECs) in hypoxic conditions, we transfected miR-210-3p mimic to overexpress miR-210-3p in human umbilical vein endothelial cells. We than performed RNA sequencing of miR-210-3p mimic-transfected and control HUVECs under hypoxic conditions to evaluate the transcriptional changes in the miR-210-3p-overexpressing HUVECs.
Project description:We quantified differential microRNA (miRNA) expression in Human umbilical vein endothelial cells (HUVECs)response to Angiogenin (ANG) treatment.These data were used to determine which miRNAs are altered on ANG in Human umbilical vein endothelial cells.
Project description:Human Umbilical Vein Endothelial Cells (HUVECs) were isolated from umbilical chords by collagenase digestion and cultured to passage 3. HUVECs were pre-activated for 4 hours with TNF-alpha (10ng/ml), followed by rHDL (0.1 and 1mg/ml) treatment for a further 8 hours. RNA isolated and labeled using the Illumina total prep RNA amplification kit and analysed using the Illumina sentrix beadchip microarray HT-12 (n=3 per treatment)
Project description:HRG is a 75kDa heparin-binding protein non to extert gene regultaion changes on macropahges. To assess the effect of HRG on Enodothelial Cell gene regulation Human Umbilical Vein Endothelial Cells (HUVECs) were treated with Histidine-Rich Glycoprotein (HRG) 24 hour treatment with HRG. and examined for gene regulation changes versus untreated HUVECs after 6 and