Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Saccharomyces cerevisiae

ABSTRACT: ChIP-Seq of constitutively expressed Ndt80-Myc in S. cerevisiae

PROVIDER: PRJNA355297 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5061144.fastq.gz	Fastqsanger.gz
	SRR5061145.fastq.gz	Fastqsanger.gz
	SRR5061146.fastq.gz	Fastqsanger.gz
	SRR5061147.fastq.gz	Fastqsanger.gz
	SRR5061148.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

ChIP-Seq of constitutively expressed Ndt80-Myc in S. cerevisiae

Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in S. cerevisiae when constitutively expressed, for the native Ndt80 as well as a heterologous Ndt80 from Pichia pastoris. For the ChIPs on the native Ndt80s, Ndt80 was tagged with c-myc; for the heterologous ChIP P. pastoris Ndt80 was integrated into the S. cerevisiae genome and tagged with c-myc. In both cases, the native Ndt80 promoter was also replaced with an A. gossypii Gal1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown overnight in SRaffinose until log-phase, then in SRaffinose + 1.7% Galactose for 5 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.

2017-05-01 | GSE90660 | GEO

Saccharomyces cerevisiae

Project description:ChIP-Seq of Ndt80-Myc in S. cerevisiae during meiosis and sporulation

| PRJNA355296 | ENA

Komagataella pastoris

Project description:ChIP-Seq of constitutively expressed Ndt80-Myc in P. pastoris

| PRJNA355300 | ENA

Kluyveromyces lactis

Project description:ChIP-Seq of constitutively expressed Ndt80-Myc in K. lactis

| PRJNA355301 | ENA

ChIP-Seq of Ndt80-Myc in S. cerevisiae during meiosis and sporulation

Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in S. cerevisiae during meiosis and sporulation. Ndt80 was tagged with c-myc and the protein was immunoprecipitated with a c-myc antibody. Cells were grown in liquid YPA (2% Peptone, 2% Potassium Acetate, 1% Yeast Extract) at room temperature for 22 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.

2017-05-01 | GSE90661 | GEO

ChIP-Seq of constitutively expressed Ndt80-Myc in P. pastoris

Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in P. pastoris when constitutively expressed. Ndt80 was tagged with c-myc, the native Ndt80 promoter was replaced with the E. gossypii Tef1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown unti log-phase in YPD. Each experiment was repeated twice and sequenced on an Illumina HiSeq 2500.

2017-05-01 | GSE90663 | GEO

ChIP-Seq of constitutively expressed Ndt80-Myc in K. lactis

Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in K. lactis when constitutively expressed. Ndt80 was tagged with c-myc, the native Ndt80 promoter was replaced with the K. lactis Gal1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown overnight in SRaffinose until log-phase, then in SRaffinose + 1.7% Galactose for 5 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.

2017-05-01 | GSE90662 | GEO

S-phase independent silencing establishment in Saccharomyces cerevisiae

Project description:As part of a study of establishment of silencing in Saccharomyces cerevisiae, we performed ChIP-seq on myc-tagged Sir4 in several conditions. Included in those conditions are wild-type cycling cells, cycling sir3∆ cells, and various experiments during which silencing establishment was controlled using the inducible SIR3-EBD allele. Silencing establishment experiments were performed in both wild-type and dot1∆ cells.

2020-05-19 | GSE150737 | GEO

ChIP-Seq of constitutively expressed Ndt80A-Myc and Ndt80B-Myc in S. stipitis

Project description:The purpose of this experiment was to identify the genes bound by the two Ndt80 paralogs in S. stipitis when constitutively expressed. Both paralogs were tagged with c-myc, the native Ndt80 promoter was replaced with the E. gossypii Tef1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown unti log-phase in YPD. Each experiment was repeated twice and sequenced on an Illumina HiSeq 2500.

2017-05-01 | GSE90664 | GEO

ChIP-Seq of constitutively expressed Ndt80A-Myc and constitutively and endogenously expressed Ndt80B-Myc in C. albicans

Project description:The purpose of this experiment was to identify the genes bound by the two Ndt80 paralogs in C. albicans when constitutively expressed, and for Ndt80B when endogenously expressed. Both paralogs were tagged with c-myc, and the protein was immunoprecipitated with a c-myc antibody. For constitutive expression, each native Ndt80 promoter was replaced by the C. albicans Tdh3 promoter. Cells were grown unti log-phase in YPD. Each experiment was repeated twice and sequenced on an Illumina HiSeq 2500.

2017-05-01 | GSE90665 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data