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Pseudomonas aeruginosa PA14

ABSTRACT: Experimental evolution of high levels antibiotic resistance and collateral sensitivity in Pseudomonas aeruginosa

PROVIDER: PRJNA355367 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5363640.fastq.gz	Fastqsanger.gz
	SRR5363640_1.fastq.gz	Fastqsanger.gz
	SRR5363640_2.fastq.gz	Fastqsanger.gz
	SRR5363650.fastq.gz	Fastqsanger.gz
	SRR5363650_1.fastq.gz	Fastqsanger.gz

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Pseudomonas aeruginosa PA14

Project description:Experimental evolution of antibiotic resistance with Pseudomonas aeruginosa PA14

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Lethal Antibiotic Exposure Drives the Convergent Evolution of High Tolerance Phenotype Conferred by nfxB Mutation in clinically relevant Pseudomonas aeruginosa

Project description:Fluoroquinolone antibiotics, a common antibiotic for the treatment of Pseudomonas aeruginosa infection, are facing challenges due to the rapid evolution of bacterial resistance. Through designed evolutionary experiments in vitro, we find that there are significant differences in evolutionary trajectories and outcomes of resistant bacteria obtained in different induction modes, among which fitness benefit of resistant strains obtained in high-dose induction mode under high level of antibiotic is significantly higher than that of wild-type strain, and collateral sensitivity to aminoglycosides and some other antibiotics will be obtained. Resistance strains obtained in the low-dose induction mode exhibit higher heterogeneity, which is accompanied by multiple drug resistance (MDR). Through second generation resequencing and proteomic techniques, overexpression of MexCD-OprJ efflux pump induced by mutations in the nfxB gene significantly improved the fitness benefit of Pseudomonas aeruginosa PAO1 under high level of fluoroquinolones. This is the precondition of the further evolution of the fleroxacin-resistant strains in the high dose induction mode, the addition of the efflux pump inhibitor phenylalanyl arginyl β-naphthylamide (PAβN) could effectively repress the evolution of bacterial resistance in the high dose induction mode. Fleroxacin use followed by gentamicin helped drive infectious P.aeruginosa to extinction, causing nfxB mutation to cause collateral sensitivity to gentamicin.

2025-02-15 | PXD044054 | Pride

Phenotypic convergent evolution towards fosfomycin collateral sensitivity of Pseudomonas aeruginosa antibiotic resistant mutants

Project description:Phenotypic convergent evolution towards fosfomycin collateral sensitivity of Pseudomonas aeruginosa antibiotic resistant mutants

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Genomic DNA hybridization of Pseudomonas aeruginosa strains PAO1 and PA14

Project description:Genomic DNA from Pseudomonas aeruginosa strains PAO1 and PA14

2015-11-15 | GSE67038 | GEO

Proteomics of Pseudomonas aeruginosa PAO1 and PA14

Project description:Recent studies have shown that the concentrations of proteins expressed from orthologous genes are often conserved across organisms, and to a greater extent than the abundances of the corresponding mRNAs. However, such studies have not distinguished between evolutionary (e.g., sequence divergence) and environmental (e.g., growth condition) effects on the regulation of steady-state protein and mRNA abundances. Here we systematically investigated the transcriptome and proteome of two closely related Pseudomonas aeruginosa strains, PAO1 and PA14, under identical experimental conditions, thus controlling for environmental effects. For 703 genes observed by both shotgun proteomics and microarray experiments, we find that the protein-to-mRNA ratios are highly correlated between orthologous genes in the two strains, to an extent comparable to protein and mRNA abundances. In spite of this high molecular similarity between PAO1 and PA14, we found that several metabolic, virulence, and antibiotic resistance genes are differentially expressed between the two strains, mostly at the protein but not at the mRNA level. Our data demonstrate that post-transcriptional regulation is important for understanding the discordance between mRNA and protein abundance.

2014-05-08 | PXD000684 | Pride

Phenotypic convergent evolution towards fosfomycin collateral sensitivity of Pseudomonas aeruginosa antibiotic resistant mutants

Project description:In this work we describe a robust fosfomycin collateral sensitivity phenotype of Pseudomonas aeruginosa resistant mutants selected by antibiotics from different structural families. The underlying mechanism was the reduced expression of the genes encoding the peptidoglycan-recycling pathway, which preserves the peptidoglycan synthesis in situations where the de novo synthesis is blocked, and of fosA, encoding a fosfomycin-inactivating enzyme.

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Transcriptional modulation of Quoring-sensing signaling throught QslA in Pseudomonas aeruginosa PA14 and PAO1.

Project description:To further determine the origin of the increased virulence of Pseudomonas aeruginosa PA14 compared to Pseudomonas aeruginosa PAO1, we report a transcriptomic approach through RNA sequencing. Next-generation sequencing (NGS) has revolutioned sistems-based analsis of transcriptomic pathways. The goals of this study are to compare the transcriptomic profile of all 5263 orthologous genes of these nearly two strains of Pseudomonas aeruginosa.

2019-09-23 | GSE125646 | GEO

topA mutant of P. aeruginosa PA14

Project description:We have tested the effect of deletion of Topoisomerase I on genes transcription of the bacteria Pseudomonas. aeruginosa. Topoisomerase I is a conserved protein that modulate the topology state of the DNA from super-coiled to relaxed. To this end we have compared the transcription profile of a topA::GentR mutant to that of the wild-type strain. The topA mutant contains in topA gene a Mariner transposon with a gentamicin resistance cassette. It is part of the non-redundant mutant library of P. aeruginosa PA14 strains.

2011-12-21 | GSE24038 | GEO

Transcriptome Sequence of Antibiotic-Treated Pseudomonas aeruginosa

Project description:Pseudomonas aeruginosa is known to tolerate antibiotic therapy during infection. This prevents clearance of infection and negatively impacts patient outcomes. Here, we report the transcriptome sequence of antibiotic-treated and untreated P. aeruginosa cultures and the differential gene expression observed when treated cells are compared to untreated cells.

2018-10-31 | GSE120602 | GEO

Pseudomonas aeruginosa PA14

Project description:Pseudomonas aeruginosa PA14 long term evolution in acidic pH

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