Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA355547 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Plants reorganize their root architecture to avoid growth into unfavorable regions of the rhizosphere. In a screen based on chimeric repressor gene-silencing technology, we identified the Arabidopsis thaliana GeBP-LIKE 4 (GPL4) transcription factor as an inhibitor of root growth that is induced rapidly in root tips in response to cadmium (Cd). We tested the hypothesis that GPL4 functions in the root avoidance of Cd by analyzing root proliferation in split medium, in which only half of the medium contained toxic concentrations of Cd. The wild-type (WT) plants exhibited root avoidance by inhibiting root growth in the Cd side but increasing root biomass in the control side. By contrast, GPL4-suppression lines exhibited nearly comparable root growth in the Cd and control sides and accumulated more Cd in the shoots than did the WT. GPL4 suppression also altered the root avoidance of toxic concentrations of other essential metals, modulated the expression of many genes related to oxidative stress, and consistently decreased reactive oxygen species concentrations. We suggest that GPL4 inhibits the growth of roots exposed to toxic metals by modulating reactive oxygen species concentrations, thereby allowing roots to colonize noncontaminated regions of the rhizosphere.thereby re-allocating root biomass toward non-contaminated rhizosphere areas and minimizing root exposure to toxic metals.
2016-12-01 | GSE90701 | GEO
Project description:Transcription profiling of Arabidopsis thaliana root tip region comparing argonaute1-101 (SALK_035319), scr-3 and wild-type Col-0.
2009-12-31 | GSE16460 | GEO
Project description:Cadmium (Cd) is a significant environmental pollutant with widespread detrimental effects on living organisms, making it a frequent subject of laboratory studies. However, different types of Cd salts are used to spike media, often without considering the possibility that accompanying anions may influence the effects of metal cations. Using two commonly used Cd salts, CdSO4 and CdCl2, we observed distinct toxicity effects on Arabidopsis thaliana development. On a physiological level, 7-day-old seedlings exposed to 50 µM CdSO4 had shorter roots than those treated with CdCl2. Proteomic analysis revealed strong downregulation of proteins involved in microtubule organization and primary cell wall synthesis in the root of plants exposed to CdSO4. Additionally, these plants exhibited higher Cd uptake from the medium and greater Cd accumulation in the shoot, indicating that the SO42-, as an accompanying anion, exacerbates Cd toxicity. These findings highlight the critical but often overlooked role of accompanying anions in modulating the toxic effects of heavy metals on plants.
2026-01-07 | PXD062135 | Pride
Project description:Shade avoidance syndrome (SAS) is a strategy of major adaptive significance that includes the elongation of vegetative structures and leaf hyponasty. Major transcriptional rearrangements underlie for the reallocation of resources to elongate vegetative structures and redefine the plant architecture under shade to compete for photosynthesis light. BBX28 is a transcription factor involved in seedling de-etiolation and flowering in Arabidopsis thaliana, but its function in the SAS is completely unknown. Here we studied the function of BBX28 in the regulation of gene expression under simulated shade conditions.
2024-03-15 | GSE217723 | GEO
Project description:We characterised mutants in the GRAS family transcription factor AtSCL26 in Arabidopsis thaliana using a combination of gene functional analysis, hormone treatments and expression profiling at the cell type level. This has enabled us to implicate AtSCL26 in the cell-specific control of nitrogen-giberellic acid response cross-talk to control root architecture.
2023-01-27 | GSE58046 | GEO
Project description:Sequence-specific transcription factor WRKY75 is highly responsive to reactive oxygen species on transcriptional level in the rosettes of Arabidopsis thaliana. In addition, it acts in developmental responses, acquisition of nutrients, and in stress responses. In the root, WRKY75 is a repressor of root hair formation, it regulates the phosphate starvation response, and the response to certain pathogens. In order to find the target genes of WRKY75, the effects of estradiol-inducible overexpression of WRKY75 on transcriptome was studied using RNA-seq.
2016-04-03 | GSE79856 | GEO
Project description:For shade-intolerant species, shade light indicates the close proximity of neighboring plants and triggers the shade avoidance syndrome (SAS), which causes exaggerated growth and reduced crop yield. We report that microbiotal root commensals(Pseudomonas fluorescens and Root918) alleviate the shade avoidance responses in Arabidopsis. To identify the functions of Pseudomonas fluorescens and Root918 during SAS, we performed RNA-seq to search for differentially expressed genes (DEGs) in different tissues by comparing the transcript levels of shoot and root parts of col0 in germ-free, Pseudomonas fluorescens and Root918 during white light and shade conditions.
2025-05-11 | GSE263116 | GEO
Project description:Transcription factor transcriptome during somatic embryogenesis (SE) in Arabidopsis thaliana
| PRJNA195892 | ENA
Project description:CrY2H-seq Arabidopsis Thaliana transcription factor screen raw sequence reads
| PRJNA386337 | ENA
Project description:Oxygen is a key signalling component of plant biology and, whilst an oxygen-sensing mechanism was previously described in Arabidopsis thaliana. However, key features of the associated PCO N-degron pathway and Group VII ETHYLENE RESPONSE FACTOR (ERFVII) transcription factor substrates remain unknown. We demonstrate that ERFVIIs show non-autonomous activation of root hypoxia tolerance, and are essential for root development and survival under oxygen limiting conditions in the soil. We determine the combined effects of ERFVIIs in controlling genome expression and define genetic and environmental components required for proteasome-dependent oxygen-regulated stability of ERFVIIs through the PCO N-degron pathway. Using a plant extract, unexpected amino-terminal cysteine oxidation to sulphonic acid oxidation level of ERFVIIs was defined, suggesting a requirement for additional enzymatic activity within the pathway. Our results provide a holistic understanding of the properties, functions and readouts of this oxygen-sensing mechanism defined through its role in modulating ERFVII stability.
2023-07-02 | PXD039288 | Pride