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Sorghum sudanense

ABSTRACT: Sorghum sudanense under grought stress. Transcriptome

PROVIDER: PRJNA359376 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5183088.fastq.gz	Fastqsanger.gz
	SRR5183088_1.fastq.gz	Fastqsanger.gz
	SRR5183088_2.fastq.gz	Fastqsanger.gz

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Sorghum sudanense

Project description:Sorghum sudanense under grought stress. Transcriptome

| PRJNA359377 | ENA

Genome-wide profiling of small RNAs in sorghum under abiotic stresses

Project description:To identify novel miRNA and NAT-siRNAs that are associated with abiotic stresses in sorghum, we generated small RNA sequences from sorghum seedlings that grew under control and under dought, salt, and cold stress treatments.

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Project description:Salt stress has become one of the main abiotic stress factors restricting agricultural production worldwide. Sweet sorghum is an important salt and drought tolerant feed and energy crop. Its salt tolerance mechanism has not been widely studied. With the development of transcriptome sequencing technology, it is possible to study the molecular mechanism of sweet sorghum salt tolerance. The purpose of this study was to further reveal the potential salt-tolerant molecular mechanisms of sweet sorghum through high-throughput sequencing analysis of the transcriptome. Finally, through high-throughput sequencing, we read approximately 54.4G of raw base and 53.7G of clean base in total, and used FastQC to assign a quality score (Q) to each base in the read using a similar phred algorithm, Analysis shows that the data is highly credible. We conclude that RNA-based transcriptome characterization will accelerate the study of genetics and molecular biology of sweet sorghum salt tolerance mechanisms and provide a framework for this.

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small RNAs sequencing in sweet sorghum under cadmium stress

Project description:Four small RNA libraries from two contrasting sweet sorghum genotypes were sequenced. In this study, One hundred and ninety-five conserved miRNAs belonging to 56 families and 25 putative novel miRNAs from 28 precursors were identified, among which 38 conserved and 24 novel miRNAs were differentially expressed under Cd stress and/or between H18 and L69. Two groups of them: miR169p/q-nov_23 and miR408 were further focused through the coexpression analysis and might be involved in Cd transport, cytoskeleton activity and cell wall construction by regulating their targets. This study presents new insights into the regulatory roles of miRNAs in Cd accumulation and tolerance in sweet sorghum and will help to develop high-Cd accumulation or high Cd-resistant germplasm of sweet sorghum through molecular breeding and/or genetic engineering approaches.

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Project description:To verify whether phosphorus deficiency can induce sorghum to produce and secrete SLs, we conducted RNA-sequencing (RNA-seq) analyses in sorghum plants grown under phosphorus deficiency conditions; to verify which genes induced by SL treatment, we conducted RNA-sequencing (RNA-seq) analyses in sorghum plants grown under SL treatment.

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Project description:This study utilized next generation sequencing technology (RNA-Seq) to examine the transcriptome of sorghum plants challenged with osmotic stress and exogenous abscisic acid (ABA) to elucidate those genes and gene networks that contribute to sorghum's tolerance to water-limiting environments with a long-term aim of developing strategies to improve plant productivity under drought. We examined the mRNA of 9 day old Sorghum bicolor (BTx623) from 2 tissue types (roots and shoots) for 2 treatments (20 uM ABA and 20% PEG) with corresponding controls (0.2M NaOH and H2O) for 27 hrs prior to harvesting, each done in triplicate biological replicates - resulting in 24 unique runs

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2012-04-08 | GSE36689 | GEO

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