Project description:Liver samples were isolated from postnatal day 15 (P15) untreated mice and then flash frozen: Mus musculus musculus (C57BL/6J), Mus musculus castaneus (CAST), and Mus caroli (CAROLI). DEN-induced liver tumours and background liver tissue were collected from 37-week old C57BL/6J mice. ATAC-seq was performed to identify the open regions of the genome.
Project description:Microglial replacement is emerging as a promising approach for treating age-related disorders, but effects on epigenetic age remain unclear. We examined DNA methylation changes in mouse microglia influenced by age and microglial replacement using Infinium Mouse Methylation BeadChips. We analyzed the FACS-sorted microglia of young (N=4), old (N=4) and old with microglial replacement (N=4) achieved using a PLX diet. Old microglia showed an aged epigenetic profile. Microglial depletion/repopulation, driving proliferation, led to accelerated epigenetic age. However, at the genome-wide level, repopulation reversed many aging-related changes, particularly those linked to immune and inflammatory response.
